Glyphosate-induced lipid metabolism disorder contributes to hepatotoxicity in juvenile common carp. (15th January 2021)

• Record Type: Journal Article
• Authors: Liu, Jingbo; Dong, Chenyu; Zhai, Zhenzhen; Tang, Liang; Wang, Lin
• Is Part Of: Environmental pollution. Volume 269(2021)
• Publication Date: 2021-01-15
• Subjects: Glyphosate -- Common carp -- Oxidative stress -- Inflammation -- Lipid metabolism disorder; ACC acetyl-CoA carboxylase -- ALT alanine transaminase -- AST aspartate transaminase -- CAT catalase -- CPT carnitine palmitoyltransferase -- DCFH-DA 7′-dichloro-dihydro-fluorescein diacetate -- FAS fatty acid synthetase -- FFAs free fatty acids -- GLY glyphosate -- GSH-Px glutathione peroxidase -- HDL-C high-density lipoprotein cholesterol -- HO-1 Heme oxygenase-1 -- HSL hormone sensitive triglyceride lipase -- IL-1β interleukin-1β -- IL-6 interleukin-6 -- IL-8 interleukin-8 -- IL-10 interleukin-10 -- LDL-C low-density lipoprotein cholesterol -- LPL lipoprotein lipase -- MDA malondialdehyde -- NQO1 NADH quinone oxidoreductase 1 -- Nrf-2 nuclear factor erythroid-2 related factor 2 -- PCR polymerase chain reaction -- PPAR-α peroxisome proliferator-activated receptor-α -- PPAR-γ peroxisome proliferator-activated receptor-γ -- ROS reactive oxygen species -- SOD superoxide dismutase -- SREBP1 sterol regulatory-element binding protien-1 -- TC total cholesterol -- TG triglyceride -- TMS tricaine methane sulfonate -- TNF-α tumor necrosis factor-α -- TNF-β tumor necrosis factor-β; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.116186
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Residues of glyphosate (GLY) are widely detected in aquatic systems, raising potential environmental threats and public health concerns, but the mechanism underlying GLY-induced hepatotoxicity in fish has not been fully elucidated yet. This study was designed to explore the hepatotoxic mechanism using juvenile common carp exposed to GLY for 45 d, and plasma and liver samples were collected at 15 d, 30 d, and 45 d to analyze the assays. First, GLY-induced hepatic damage was confirmed by serum liver damage biomarker and hepatic histopathological analysis. Next, changes in oxidative stress biomarkers, gene expression levels of pro- and anti-inflammatory cytokines, and lipid metabolism-related parameters in collected samples were analyzed to clarify their roles in GLY-induced hepatic damage. Data showed that oxidative stress was an early event during GLY exposure, followed by hepatic inflammatory response. Lipid metabolism disorder was a late event during GLY exposure, as evidenced by overproduced hepatic free fatty acids, enhanced lipogenesis-related gene expression levels, reduced lipolysis-related gene expression levels, and resultant hepatic lipid accumulation. Collectively, these findings demonstrate that GLY induces hepatotoxicity in fish through involvement of oxidative stress, inflammatory response, and lipid metabolism disorder, which are intimately interrelated with each other during GLY exposure. Graphical abstract: Image 1 Highlights: Sub-chronic glyphosate (GLY) exposure induces hepatic damage in fish. Oxidative stress and resultant inflammatory response are involved in GLY-exposed fish. GLY-induced inflammation contributes to hepatic lipid metabolism disorder in fish. GLY-induced hepatotoxicity in fish is due to lipid metabolic disorders.