An evaluation of the pharmacokinetics of tiotropium following a single-dose inhalation in healthy subjects using an ultra-sensitive bioanalytical method. (2nd December 2019)

• Record Type: Journal Article
• Authors: Bouhajib, Mohammed; Tayab, Zia
• Is Part Of: Drug development and industrial pharmacy. Volume 45:Number 12(2019)
• Page Start: 1915
• Page End: 1920
• Publication Date: 2019-12-02
• Subjects: Tiotropium -- pharmacokinetics -- lower limit of quantitation -- sensitivity -- inhalation; Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.1080/03639045.2019.1680993
• Languages: English
• ISSNs: 0363-9045

Abstract

Abstract: Objective and significance: The systemic bioavailability of tiotropium following administration via inhalation is known to be very low. A validated ultra-sensitive bioanalytical method with the lowest lower limit of quantitation (LLOQ) was developed and used to evaluate the complete pharmacokinetic profile of tiotropium. Methods: This was a pharmacokinetic study performed in 18 healthy subjects. Each subject was administered a dose of 18 mcg of tiotropium from a dry powder inhaler (DPI). The subjects' plasma tiotropium concentrations were assayed with LLOQ of 0.1 pg/mL. Results: The results showed a mean C max of 4.98 ± 3.55 pg/mL, and a median ( t max ) of 3.6 minutes (range: 1.8–12 minutes). The means for area under the concentration–time curve (AUC) from time zero hours to infinity (AUCinf ) and AUC from time zero hours to the time of the last measurable tiotropium concentration (AUC t ) were 51.11 ± 27.4 pg*h/mL and 37.37 ± 23.38 pg*h/mL, respectively. The mean apparent elimination half-life ( t 1/2 ) was 68.02 ± 24.55 hours. This calculated half-life is longer than what others have reported where a less sensitive LLOQ was used. Conclusion: The lower LLOQ enabled further insight into the pharmacokinetics of tiotropium that was not possible with other analytical methods. With this method, we were able to quantify tiotropium concentrations as early as one minute following drug administration and up to 144 hours after dosing. The application of this method will allow for studies to be designed properly and enable further investigations into the pharmacokinetics of tiotropium.