A novel route for aspartame production by combining enzymatic and chemical reactions for industrial use. Issue 2 (31st December 2020)

• Record Type: Journal Article
• Authors: Yokozeki, Kenzo; Abe, Isao
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 2(2021)
• Page Start: 464
• Page End: 466
• Publication Date: 2020-12-31
• Subjects: α-amino acid ester acyltransferase -- aspartame production, l-aspartic acid α, β-dimethylester -- l-phenylalanine -- α-l-aspartyl-l-phenylalanine β-methylester; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbaa045
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Here, we report a novel industrial aspartame production route, involving the enzymatic production of α-l -aspartyl-l -phenylalanine β-methylester from l - aspartic acid dimethylester and l -phenylalanine by α-amino acid ester acyl transferase. The route also involves the chemical transformation of α-l -aspartyl-l -phenylalanine β-methylester to α-l -aspartyl-l -phenylalanine methylester hydrochloride (aspartame hydrochloride) in an aqueous solution with methanol and HCl, followed by HCl removal to form aspartame.