A new peptaibol, RK-026A, from the soil fungus Trichoderma sp. RK10-F026 by culture condition-dependent screening. Issue 1 (7th January 2021)

• Record Type: Journal Article
• Authors: Rawa, Mira Syahfriena Amir; Nogawa, Toshihiko; Okano, Akiko; Futamura, Yushi; Nakamura, Takemichi; Wahab, Habibah A; Osada, Hiroyuki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 1(2021)
• Page Start: 69
• Page End: 76
• Publication Date: 2021-01-07
• Subjects: peptaibols -- cytotoxicity -- K562 -- fungi -- natural product; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbaa051
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: A new peptaibol, RK-026A (1 ) was isolated from a fungus, Trichoderma sp. RK10-F026, along with atroviridin B (2 ), alamethicin II (3 ), and polysporin B (4 ) as a cytotoxic compound, which was selected by principal component analysis of the MS data from 5 different culture conditions. The structure of 1 was determined as a new atroviridin B derivative containing Glu at the 18th residue instead of Gln by NMR and HR-MS analyses including the investigation of detailed MS/MS fragmentations. 1 showed cytotoxicity toward K562 leukemia cells at an IC50 value of 4.1 µm . Graphical Abstract: