An acetylated mannan isolated from Aloe vera induce colorectal cancer cells apoptosis via mitochondrial pathway. (1st September 2022)

• Record Type: Journal Article
• Authors: Tong, Xueli; Lao, Chunqin; Li, Di; Du, Junxi; Chen, Jingmian; Xu, Weijie; Li, Lu; Ye, Huiling; Guo, Xiaofeng; Li, Jiejing
• Is Part Of: Carbohydrate polymers. Volume 291(2022)
• Publication Date: 2022-09-01
• Subjects: AVBEC Aloe vera barbadensis extract C -- ABPA1 aloe barbadensis polymeric acemannan 1 -- ATP adenosine triphosphate -- AP aloe polysaccharide -- BSA bull serum albumin -- Cyto-C Cytochrome-C -- DAPI diamidinyl phenyl indole -- DCFH-DA 2, 7-Dichlor-odihydro-fluorescein diacetate -- EDTA ethylene diamine tetraacetie acid -- DAB diaminobenzidine -- ECL electrochemiluminescence -- ETC electron transport chain -- FITC fluorescein isothiocyanate -- FADH2 flavine adenine dinucleotide reduced -- GLP good laboratory practices -- GC–MS gas chromatography-mass spectrometer -- HPGPC high-performance gel permeation chromatography -- HRP horseradish peroxidase -- HE hematoxylin eosin -- IHC immunohistochemistry -- NMR nuclear magnetic resonance -- NADH nicotinamide adenine dinucleotide -- OXPHOS oxidative phosphorylation -- PSK polysaccharide Kreha -- PVDF polyvinylidene fluoride -- PBS phosphate buffer saline -- ROS reactive oxygen species -- SPF specific pathogen free -- SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis -- TUNEL TdT-mediated dUTP nick end labeling -- TMRE tetramethylrhodamine ethyl ester -- TEM transmission electron microscopy -- TCA tricarboxylic acid; Aloe polysaccharide -- Colorectal cancer -- Mitochondria -- Apoptosis -- Metabolism; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119464
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: The anti-cancer effects of Aloe vera barbadensis extract C (AVBEC) have been demonstrated in a previous study. However, the specific functional ingredient and mechanism remain undefined. This study aimed to evaluate the function and associated mechanisms of purified polysaccharide (ABPA1) from AVBEC on colorectal cancer. Here, we identify that ABPA1 can induce colorectal cancer apoptosis. In vivo, ABPA1 significantly suppressed tumor growth in an orthotopic colon cancer model. Mechanistically, ABPA1 alters mitochondrial membrane permeability by promoting Bax translocation while causing cytochrome-c release, which initiates the caspase cascade reaction. Additionally, we found that ABPA1 exerted distinct impacts on the mitochondrial metabolism of colorectal cancer cells. Our study elucidated the mechanism by which the polysaccharide ABPA1 induces apoptosis in colorectal cancer cells through the regulation of Bax and cytochrome-c mediated mitochondrial pathway, indicating that ABPA1 may be developed as a mitochondrial-targeting anti-cancer drug. Graphical abstract: Unlabelled Image