Metabolic changes in cucumber leaves are enhanced by blue light but differentially affected by UV interactions with light signalling pathways in the visible spectrum. (August 2022)

• Record Type: Journal Article
• Authors: Palma, Carolina Falcato Fialho; Castro-Alves, Victor; Morales, Luis Orlando; Rosenqvist, Eva; Ottosen, Carl-Otto; Hyötyläinen, Tuulia; Strid, Åke
• Is Part Of: Plant science. Volume 321(2022)
• Publication Date: 2022-08
• Subjects: Ultraviolet radiation -- LEDs -- Light quality -- Cucumber -- Metabolome -- Metabolic regulation; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2022.111326
• Languages: English
• ISSNs: 0168-9452

Abstract

Abstract: Ultraviolet radiation (UV, 280–400 nm) as an environmental signal triggers metabolic acclimatory responses. However, how different light qualities affect UV acclimation during growth is poorly understood. Here, cucumber plants ( Cucumis sativus ) were grown under blue, green, red, or white light in combination with UV. Their effects on leaf metabolites were determined using untargeted metabolomics. Blue and white growth light triggered increased levels of compounds related to primary and secondary metabolism, including amino acids, phenolics, hormones, and compounds related to sugar metabolism and the TCA cycle. In contrast, supplementary UV in a blue or white light background decreased leaf content of amino acids, phenolics, sugars, and TCA-related compounds, without affecting abscisic acid, auxin, zeatin, or jasmonic acid levels. However, in plants grown under green light, UV induced increased levels of phenolics, hormones (auxin, zeatin, dihydrozeatin-7-N-dihydrozeatin, jasmonic acid), amino acids, sugars, and TCA cycle-related compounds. Plants grown under red light with UV mainly showed decreased sugar content. These findings highlight the importance of the blue light component for metabolite accumulation. Also, data on interactions of UV with green light on the one hand, and blue or white light on the other, further contributes to our understanding of light quality regulation of plant metabolism. Highlights: The metabolite profile of cucumber leaves is dependent on growth light quality. Plants grown under blue or white had high metabolite levels, under green or red low. UV responses are highly dependent on the quality of the growth light background. Supplemental UV decreased metabolite content in plants grown under white or blue. Plants grown under green light had higher metabolite levels when exposed to UV.