Conductive polymer sorbent for extraction and determination of resveratrol and polydatin in Polygonum cuspidatum root samples. Issue 4 (30th January 2022)

• Record Type: Journal Article
• Authors: Xue, Xuemei; Deng, Fei; Ge, Zhenkai; Meng, Jingwen; Liu, Xiya; Zhao, Yongheng; Hu, Yanggen; Ling, Xu
• Is Part Of: Phytochemical analysis. Volume 33:Issue 4(2022)
• Page Start: 590
• Page End: 598
• Publication Date: 2022-01-30
• Subjects: carbon fiber bundles -- electropolymerization -- in‐tube solid phase microextraction -- Polygonum cuspidatum -- resveratrol and polydatin; Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.3112
• Languages: English
• ISSNs: 0958-0344

Abstract

Abstract: Introduction: The quantitative analysis of trace resveratrol and polydatin in plant tissues is suitable for elucidation of the compounds' mechanisms of action. Objectives: The main objective of this work was to develop a feasible and effective sample pretreatment method to measure the concentrations of resveratrol and polydatin in complex samples. Methodology: A polymer sorbent, poly(2‐mercaptobenzimidazole), was electrochemically prepared and utilized for selective extraction, while resveratrol and polydatin were used as target analytes. The sorbent was characterized by cyclic voltammetry, scanning electron microscopy and Fourier transform infrared spectroscopy. After extraction and elution, the analytes were analyzed by a Thermo U3000 HPLC system. Several affecting parameters, including the volume of elution solution, sample pH value, sample flow rate and sample volume, were evaluated and optimized. Results: The proposed method showed good linearity with low limits of detection (from 0.5 to 0.8 ng·mL −1 ) and ideal accuracy with spiked recoveries from 81.30% to 99.16%. A good enrichment factor (more than 200‐fold) together with good sensitivity was obtained with this method. Analysis of resveratrol and polydatin in Polygonum cuspidatum samples by this method is efficient. Conclusion: The method developed in this work exhibits several significant merits, including easy operation and high extraction efficiency, indicating that electrochemically prepared polymer sorbent is useful for sample pretreatment and analysis of traditional Chinese medicine samples. Abstract : Carbon fiber bundles electrochemically modified with poly(2‐mercaptobenzimidazole) were packed into PEEK tube loop and connected with HPLC system. Good stability and extraction efficiency over 200‐fold for resveratrol and polydatin was achieved with the system. Application in plant samples exhibits high extraction selectivity, good reproducibility and recoveries.