Blocking the Cu (II) Ions Mediated Catalytical Ability for Construction of Ratiometric Fluorescence Sensing Platform Based on Glutathione-Stabilized Copper Nanoclusters. Issue 3 (1st March 2022)

• Record Type: Journal Article
• Authors: Tao, Bei-Bei; Wu, Ning-Ning; Zhang, Hong-Ding; Wang, Hai-Bo
• Is Part Of: Journal of the Electrochemical Society. Volume 169:Issue 3(2022)
• Publication Date: 2022-03-01
• Subjects: Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/1945-7111/ac5f1e
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : It is of great importance to integrate two kinds of fluorescent probes in one system to construct ratiometric sensing platform. Inspired by the efficient overlapped spectrum of glutathione-stabilized copper nanoclusters (GSH-CuNCs) and 2, 3-diaminophenazine (DAP), a novel and label-free ratiometric fluorescent sensor has been developed for Cu 2+ and kojic acid (KA) detection on the basis of in situ converting o -phenylenediamine (OPD) into DAP by the catalytic oxidation ability of Cu 2+ . The Cu 2+ catalytically generated DAP was served as an energy acceptor to inhibit the fluorescence emission of GSH-CuNCs (energy donor). As a result, the sensitivity and accuracy of the strategy were greatly improved due to the dual-emission reverse change ratiometric profile. The sensing system showed high sensitivity toward Cu 2+ in a range of 0.1–50 μ M and with a limit of detection of 20 nM. Furthermore, based on the specific coordination interaction between Cu 2+ and KA, the ratiometric sensor displayed the range of detection of KA from 0.2 μ M to 300 μ M with a limit of detection of 50 nM. Thus, the ratiometric method paved a new way for the effective quantification of Cu 2+ and KA in real samples, which were closely concerned with human health.