Left Pulmonary Artery Ligation and Chronic Pulmonary Artery Banding Model for Inducing Right Ventricular—Pulmonary Hypertension in Sheep. Issue 1 (January 2021)

• Record Type: Journal Article
• Authors: Ukita, Rei; Tipograf, Yuliya; Tumen, Andrew; Donocoff, Rachel; Stokes, John W.; Foley, Neal M.; Talackine, Jennifer; Cardwell, Nancy L.; Rosenzweig, Erika B.; Cook, Keith E.; Bacchetta, Matthew
• Is Part Of: ASAIO journal. Volume 67:Issue 1(2021)
• Publication Date: 2021-01
• Subjects: pulmonary hypertension -- pulmonary artery banding -- right ventricular hypertrophy -- sheep model -- mechanical circulatory support; Artificial organs -- Periodicals; 617
• DOI: 10.1097/MAT.0000000000001197
• Languages: English
• ISSNs: 1058-2916

Abstract

Abstract : Pulmonary hypertension (PH) is a progressive disease that leads to cardiopulmonary dysfunction and right heart failure from pressure and volume overloading of the right ventricle (RV). Mechanical cardiopulmonary support has theoretical promise as a bridge to organ transplant or destination therapy for these patients. Solving the challenges of mechanical cardiopulmonary support for PH and RV failure requires its testing in a physiologically relevant animal model. Previous PH models in large animals have used pulmonary bead embolization, which elicits unpredictable inflammatory responses and has a high mortality rate. We describe a step-by-step guide for inducing pulmonary hypertension and right ventricular hypertrophy (PH-RVH) in sheep by left pulmonary artery (LPA) ligation combined with progressive main pulmonary artery (MPA) banding. This approach provides a controlled method to regulate RV afterload as tolerated by the animal to achieve PH-RVH, while reducing acute mortality. This animal model can facilitate evaluation of mechanical support devices for PH and RV failure. Abstract : Supplemental Digital Content is available in the text.