Effect of Lactobacillus casei Shirota supplementation on trimethylamine-N-oxide levels in patients with metabolic syndrome: An open-label, randomized study. Issue 1 (September 2015)

• Record Type: Journal Article
• Authors: Tripolt, Norbert J.; Leber, Bettina; Triebl, Alexander; Köfeler, Harald; Stadlbauer, Vanessa; Sourij, Harald
• Is Part Of: Atherosclerosis. Volume 242:Issue 1(2015)
• Page Start: 141
• Page End: 144
• Publication Date: 2015-09
• Subjects: Trimethylamine-N-oxide (TMAO) -- Lactobacillus casei Shirota (LcS) -- Gut microbiota -- Metabolic syndrome -- Microbiome; TMA trimethylamine -- TMAO trimethylamine-N-oxide -- LcS Lactobacillus casei Shirota -- PC phosphatidylcholine -- C choline -- CVD cardiovascular disease -- MetS metabolic syndrome -- FMO flavin monooxygenase -- sVCAM-1 soluble vascular cell adhesion molecule 1 -- sICAM-1 soluble intercellular adhesion molecule 1 -- hsCRP high-sensitivity C-reactive Protein -- HOMA-IR Homeostasis Model Assessment for Insulin Resistance -- LAC/MAN-Ratio lactulose/mannitol ratio -- DAO diaminooxidase -- CFU colony-forming unit; Arteriosclerosis -- Periodicals; Electronic journals; 616.136
• DOI: 10.1016/j.atherosclerosis.2015.05.005
• Languages: English
• ISSNs: 0021-9150

Abstract

Abstract: Background: Recent studies in animal models have shown a link between ingestion of dietary phosphatidylcholine (PC), choline, l -carnitine and cardiovascular risk. Intestinal microbiota-dependent metabolism of PC and l -carnitine is involved in formation of trimethylamine (TMA), which is further metabolized to the proatherogenic compound trimethylamine- N -oxide (TMAO). It has been suggested that changes in gut microbiota by supplementation of probiotic drinks might alter TMAO levels. Hence, the aim of this analysis was to investigate the impact of Lactobacillus casei Shirota ( Lc S) on formation of TMAO in subjects with metabolic syndrome. Methods: In a single-center, prospective, randomized-controlled study 30 subjects with metabolic syndrome were randomized to receive either 3 times daily 6.5 × 10 9 CFU (colony-forming units) Lc S (probiotic group) or not (standard therapy group) for 12 weeks. TMAO plasma levels were quantified by means of liquid chromatography and tandem mass spectrometry. Results: Thirteen patients in the probiotic group and 15 in the standard therapy group finished the study. Mean age was 52 ± 11 and 55 ± 9 years, respectively. TMAO levels decreased during the intervention period in both groups (from 4.66 ± 2.66 μM to 4.31 ± 2.04 μM in the probiotic group and from 4.64 ± 2.75 μM to 4.40 ± 2.14 μM in the control group). Changes in TMAO between subjects receiving Lc S (−0.25 ± 2.39 μM) and controls (−0.34 ± 2.23 μM) were not significantly different (p = 0.510). Conclusion: In conclusion, intake of Lc S for 12 weeks did not affect levels of TMAO in patients with metabolic syndrome.