HIV-1 Replication in HIV-Infected Individuals Is Significantly Reduced When Peripheral Blood Mononuclear Cells Are Superinfected with HSV-1. (2nd September 2012)

• Record Type: Journal Article
• Authors: Yamsuwan, Taneth; Chirathaworn, Chintana; Hansasuta, Pokrath; Bhattarakosol, Parvapan
• Other Names: Ikuta K. Academic Editor.; Maneekarn N. Academic Editor.; Zakhartchouk A. Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2012(2012)
• Publication Date: 2012-09-02
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1100/2012/102843
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : Herpes simplex virus (HSV) can cause generalized infection in human immunodeficiency virus- (HIV-) infected patients leading to death. This study investigated HSV-1 replication in PBMCs from 25 HIV-infected individuals and 15 healthy donors and the effects of HSV-1 superinfection on HIV-1 production. Herpes viral entry mediator (HVEM) receptor on T lymphocytes was also evaluated. Our results confirmed that the number of activated (CD3+ and CD38+) T lymphocytes in HIV-infected individuals (46.51 ± 17.54 %) was significantly higher than in healthy donors (27.54 ± 14.12 %, P value = 0.001) without any significant differences in HVEM expression. Even though the percentages of HSV-1 infected T lymphocytes between HIV-infected individuals (79.25 ± 14.63 %) and healthy donors (80.76 ± 7.13 %) were not different (P value = 0.922), yet HSV-1 production in HIV-infected individuals (47.34 ± 11.14 × 10 3 PFU/ml) was significantly greater than that of healthy donors (34.17 ± 8.48 × 10 3 PFU/ml, P value = 0.001). Moreover, HSV-1 virions were released extracellularly rather than being associated with the cells, and superinfection of HSV-1 at a multiplicity of infection (MOI) of 5 significantly decreased HIV production (P value < 0.001).