Hemovasculogenic origin of blood vessels in the developing mouse brain. Issue 2 (28th May 2020)

• Record Type: Journal Article
• Authors: Gama Sosa, Miguel A.; De Gasperi, Rita; Perez, Gissel M.; Hof, Patrick R.; Elder, Gregory A.
• Is Part Of: Journal of comparative neurology. Volume 529:Issue 2(2021)
• Page Start: 340
• Page End: 366
• Publication Date: 2020-05-28
• Subjects: angioblast -- angiogenesis -- hemangioblast -- hemogenic endothelium -- RRID: AB_10000854 -- RRID: AB_10598329 -- RRID: AB_2203220RRID: AB_2283583 -- RRID: AB_2811160 -- RRID: AB_305584 -- RRID: AB_306316 -- RRID: AB_476976 -- RRID: AB_477163 -- RRID: AB_726362 -- RRID: AB_870662 -- vasculogenesis primitive erythrocyte; Comparative neurobiology -- Periodicals; Neurology -- Periodicals; 616
• DOI: 10.1002/cne.24951
• Languages: English
• ISSNs: 0021-9967

Abstract

Abstract: Vascular structures in the developing brain are thought to form via angiogenesis from preformed blood vessels in the cephalic mesenchyme. Immunohistochemical studies of developing mouse brain from E10.5 to E13.5 revealed the presence of avascular blood islands of primitive erythroid cells expressing hemangioblast markers (Flk1, Tal1/Scl1, platelet endothelial cell adhesion molecule 1, vascular endothelial‐cadherin, and CD34) and an endothelial marker recognized by Griffonia simplicifolia isolectin B4 (IB4) in the cephalic mesenchyme. These cells formed a perineural vascular plexus from which angiogenic sprouts originated and penetrated the neuroepithelium. In addition, avascular isolated cells expressing primitive erythroid, hemangioblast and endothelial makers were visible in the neuroepithelium where they generated vasculogenic and hemogenic foci. From E10.5 to E13.5, these vasculogenic foci were a source of new blood vessel formation in the developing brain. In vitro, cultured E13.5 brain endothelial cells contained hemogenic endothelial cells capable of generating erythroid cells. Similar cells were present in primary cultures of dissociated cells from E10.5 embryonic head. Our results provide new evidence that the brain vasculature, like that of the yolk sac and the eye choriocapillaris and hyaloid vascular systems, develops at least in part via hemovasculogenesis, a process in which vasculogenesis and hematopoiesis occur simultaneously. Abstract : The brain vasculature develops by angiogenesis from preformed blood vessels in the cephalic mesenchyme. Here we show that in addition, avascular cells expressing primitive erythroid, hemangioblast, and endothelial makers exist in the developing cephalic mesenchyme and neuroepithelium where they generate angiogenic, vasculogenic, and hemogenic foci. From E10.5 to E13.5, the neuroepithelial vasculogenic foci were an additional source of blood vessel formation in the developing brain. Thus, the brain vasculature develops in part by hemovasculogenesis, a process in which vasculogenesis and hematopoiesis occur simultaneously.