A Cerium Vanadate Nanozyme with Specific Superoxide Dismutase Activity Regulates Mitochondrial Function and ATP Synthesis in Neuronal Cells. Issue 6 (10th December 2020)

• Record Type: Journal Article
• Authors: Singh, Namrata; NaveenKumar, Somanathapura K.; Geethika, Motika; Mugesh, Govindasamy
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 6(2021)
• Page Start: 3121
• Page End: 3130
• Publication Date: 2020-12-10
• Subjects: ATP synthesis -- cerium vanadate -- nanozymes -- oxidative stress -- superoxide dismutase; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202011711
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Nanoparticles that functionally mimic the activity of metal‐containing enzymes (metallo‐nanozymes) are of therapeutic importance for treating various diseases. However, it is still not clear whether such nanozymes can completely substitute the function of natural enzymes in living cells. In this work, we show for the first time that a cerium vanadate (CeVO4 ) nanozyme can substitute the function of superoxide dismutase 1 and 2 (SOD1 and SOD2) in the neuronal cells even when the natural enzyme is down‐regulated by specific gene silencing. The nanozyme prevents the mitochondrial damage in SOD1‐ and SOD2‐depleted cells by regulating the superoxide levels and restores the physiological levels of the anti‐apoptotic Bcl‐2 family proteins. Furthermore, the nanozyme effectively prevents the mitochondrial depolarization, leading to a significant improvement in the cellular levels of ATP under oxidative stress. Abstract : The first example of a nanozyme that can fully substitute the function of SOD1 and SOD2 in neuronal cells and regulate the synthesis of ATP under oxidative stress conditions is described. The nanozyme prevents the mitochondrial dysfunction and improves the levels of pro‐survival Bcl‐2 family proteins.