Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial Chromosome. Issue 1 (13th October 2020)

• Record Type: Journal Article
• Authors: Chiem, Kevin; Ye, Chengjin; Martinez‐Sobrido, Luis
• Is Part Of: Current protocols in microbiology. Volume 59:Issue 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-13
• Subjects: bacterial artificial chromosome -- coronavirus -- COVID‐19 -- infectious clone -- reverse genetics -- SARS‐CoV‐2 -- virus rescue; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/cpmc.126
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: SARS‐CoV‐2, the causative agent of COVID‐19, has been responsible for a million deaths worldwide as of September 2020. At the time of this writing, there are no available US FDA−approved therapeutics for the treatment of SARS‐CoV‐2 infection. Here, we describe a detailed protocol to generate recombinant (r)SARS‐CoV‐2 using reverse‐genetics approaches based on the use of a bacterial artificial chromosome (BAC). This method will allow the production of mutant rSARS‐CoV‐2—which is necessary for understanding the function of viral proteins, viral pathogenesis and/or transmission, and interactions at the virus‐host interface—and attenuated SARS‐CoV‐2 to facilitate the discovery of effective countermeasures to control the ongoing SARS‐CoV‐2 pandemic. © 2020 Wiley Periodicals LLC. Basic Protocol : Generation of recombinant SARS‐CoV‐2 using a bacterial artificial chromosome Support Protocol : Validation and characterization of rSARS‐CoV‐2