Fabrication of Aptamer‐Modified Paper Electrochemical Devices for On‐Site Biosensing. Issue 6 (10th December 2020)

• Record Type: Journal Article
• Authors: Yu, Haixiang; Chen, Zhimin; Liu, Yingzhu; Alkhamis, Obtin; Song, Zhiping; Xiao, Yi
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 6(2021)
• Page Start: 2993
• Page End: 3000
• Publication Date: 2020-12-10
• Subjects: aptamers -- biosensors -- nanomaterials -- paper electrochemical devices -- small molecules; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202008231
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Electrochemical aptamer‐based (E‐AB) sensors offer a powerful and general means for analyte detection in complex samples for various applications. Paper‐based E‐AB sensors could enable portable, low‐cost, and rapid detection of a broad range of targets, but it has proven challenging to fabricate suitable three‐electrode systems on paper. Here, we demonstrate a simple, economic, and environmentally friendly strategy for fabricating aptamer‐modified paper electrochemical devices (PEDs) via ambient vacuum filtration. The material, shape, size, and thickness of the three‐electrode PED system can be fully customized. We developed aptamer‐modified PEDs that enable sensitive and specific detection of small molecules in minimally processed biosamples. The sensitivity and stability of the PEDs are comparable to E‐AB sensors based on commercial gold electrodes. We believe our strategy can lead to the development of high performance PEDs for the on‐site detection of a variety of analytes. Abstract : A simple, economic, and environmentally friendly strategy is developed to fabricate paper electrochemical devices (PEDs) with three‐electrode systems that can be readily modified with aptamers to achieve a sensitive and specific detection of small molecules in minimally processed biosamples. Aptamer‐modified PEDs serve as a general sensing platform for the point‐of‐care detection of analytes like biomolecules and pharmaceuticals.