Anti-myocardial ischaemic effect of pseudoginsenoside F11 by inhibiting expression of beta1-adrenoceptor in rats with coronary artery ligation. (September 2017)

• Record Type: Journal Article
• Authors: Zhang, Nan-Qi; Wang, Cui-Zhu; Wang, Zhen-zhou; Li, Zhuo; Sai, Jing-Ying; Meng, Yan; Wang, Fang; Li, Ping-Ya; Liu, Jin-Ping
• Is Part Of: Journal of functional foods. Volume 36(2017)
• Page Start: 224
• Page End: 232
• Publication Date: 2017-09
• Subjects: Pseudoginsenoside F11 (PubChem CID: 71571452); Pseudoginsenoside F11 -- Anti-myocardial ischaemia -- Inhibit -- β1-AR; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.06.053
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: PF11 attenuates myocardial ischaemia injury and preserve heart function. PF11 regulates cardiac performance from inhibiting the expression of β1-AR. Molecular docking verified the binding sites between PF11 and β1-AR. Abstract: The anti-myocardial ischaemic effect of pseudoginsenoside F11 (PF11 ), a characteristic constituent of American ginseng, was evaluated in rats with coronary artery ligation. Both data and histopathological examination demonstrated that intragastric administration of PF11 at doses of 6 and 12 mg/kg could significantly improve heart function and decrease infarct size. Meanwhile, the levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST), malondialdehyde (MDA), and cardiac troponin T (cTnT) decreased, while the activity of superoxide dismutase (SOD) increased. Flow cytometry and western blotting were used to explore the underlying mechanism, and results showed that the expression of beta1-adrenoceptor (β1-AR) was decreased by PF11 . Furthermore, Glide XP software (Schrödinger) for molecular docking unravelled the probable binding sites between PF11 and β1-AR. This study provides substantial evidence for the effect of PF11 on myocardial ischaemia, and indicates that PF11 might be a potential dietary supplement for treatment of myocardial ischaemia injury.