A Sensitive Electrochemical Assay for T4 Polynucleotide Kinase Activity Based on Fe3O4@TiO2 And Gold Nanoparticles Hybrid Probe Modified Magnetic Electrode. Issue 2 (1st February 2022)

• Record Type: Journal Article
• Authors: Liu, Zaiqiong; Tao, Jinpeng; Zhu, Zhenyu; Zhang, Yanli; Wang, Hongbin; Pang, Pengfei; Wang, Haibo; Yang, Wenrong
• Is Part Of: Journal of the Electrochemical Society. Volume 169:Issue 2(2022)
• Publication Date: 2022-02-01
• Subjects: T4 polynucleotide kinase -- Fe3O4@TiO2 core–shell nanoparticles -- Gold nanoparticles -- Magnetic gold electrode -- Electrochemical sensors; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/1945-7111/ac4f6c
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : An ultrasensitive electrochemical assay for T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on magnetic Fe3 O4 @TiO2 core–shell nanoparticles and gold nanoparticles hybrid probe (Fe3 O4 @TiO2 -dsDNA-AuNPs) modified magnetic gold electrode (MGE). Fe3 O4 @TiO2 -dsDNA-AuNPs hybrid probe was formed via DNA hybridization between phosphorylated DNA S1 modified Fe3 O4 @TiO2 and complementary DNA S2 assembled AuNPs. [Ru(NH3 )6 ] 3+ was used as an electrochemically active indicator to enhance the electrochemical signal. The proposed method is sensitive for T4 PNK activity assay with a linear range of 0.0001 to 10 U ml −1 and low detection limit of 0.00003 U ml −1 . This strategy can be used to screen the T4 PNK inhibitors, holding a great potential for discovery of nucleotide kinase-target drug and early clinical diagnosis of cancer.