Coupled sulfur and electrode-driven autotrophic denitrification for significantly enhanced nitrate removal. (15th July 2022)

• Record Type: Journal Article
• Authors: Chen, Fan; Li, Zhiling; Ye, Yin; Lv, Miao; Liang, Bin; Yuan, Ye; Cheng, Hao-Yi; Liu, Yang; He, Zhangwei; Wang, Hongcheng; Wang, Yuheng; Wang, Aijie
• Is Part Of: Water research. Volume 220(2022)
• Publication Date: 2022-07-15
• Subjects: Wastewater treatment -- Sulfur autotrophic denitrification -- Electrode -- Microbial community structure -- Denitrification metabolic process -- Metagenomics; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2022.118675
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: A coupled S 0 and electrode-driven denitrification (SEAD) process was proposed. Superior NO3 − removal, less SO4 2− accumulation, and stable pH were achieved. S 0 and electrode as active bio-carriers constructed collaborative microbiome. The multi-pathway synergistic mechanisms in the SEAD process were deciphered. Metagenomic sequencing revealed the denitrification potential of S 0 and electrode. Abstract: Elemental sulfur (S 0 )-based autotrophic denitrification (SAD) has gained intensive attention in the treatment of secondary effluent for its low cost, high efficiency, and good stability. However, in practice, the supplementary addition of limestone is necessary to balance the alkalinity consumption during SAD operation, which increases water hardness and reduces the effective reaction volume. In this study, a coupled sulfur and electrode-driven autotrophic denitrification (SEAD) process was proposed with superior nitrate removal performance, less accumulation of sulfate, and self-balance of acidity-alkalinity capacity by regulating the applied voltage. The dual-channel electron supply from S 0 and electrodes made the nitrate removal rate constant k in the SEAD process 3.7-5.1 and 1.4-3.5 times higher than that of the single electrode- and sulfur-driven systems, respectively. The S° contributed to 75.3%-83.1% of nitrate removal and the sulfate yield during SEAD (5.67-6.26 mg SO4 2− /mg NO3 − -N) was decreased by 17%-25% compared with SAD. The S 0 particle and electrode both as active bio-carriers constructed collaborative denitrification communities and functional genes. Pseudomonas, Ralstonia and Brevundimonas were the dominant denitrifying genera in S 0 particle biofilm, while Pseudomonas, Chryseobacterium, Pantoea and Comamonas became dominant denitrifying genera in the cathode biofilm. The narG/Z/H/Y/I/V, nxrA/B, napA/B, nirS/K, norB/C and nosZ were potential functional genes for efficient nitrate reduction during the SEAD process. Metagenomic sequencing indicated that S 0 as an electron donor has greater potential for complete denitrification than the electrode. These findings revealed the potential of SEAD for acting as a highly efficient post denitrification process. Abstract : Image, graphical abstract