Effect of extraction methods on property and bioactivity of water-soluble polysaccharides from Amomum villosum. (6th March 2015)

• Record Type: Journal Article
• Authors: Yan, Yajuan; Li, Xia; Wan, Mianjie; Chen, Jingping; Li, Shijie; Cao, Man; Zhang, Danyan
• Is Part Of: Carbohydrate polymers. Volume 117(2015)
• Page Start: 632
• Page End: 635
• Publication Date: 2015-03-06
• Subjects: Extraction methods -- Amomum villosum -- Water-soluble polysaccharides -- Property -- Antioxidant activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.09.070
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Four extraction methods were compared for extracting water-soluble polysaccharides (WSP) from A. villosum . Samples from different extraction methods had the same monosaccharide composition. Ultrasonic-assisted extraction had the highest yield of WSP. WSP extracted by ultrasonic-assisted extraction had the best antioxidant activity. Ultrasonic-assisted extraction was the best extraction method for WSP. Abstract: In the present study, effect of different extraction methods on property and bioactivity of water-soluble polysaccharides (WSP) from the seeds of Amomum villosum were investigated. Firstly, four different extraction methods were used to extract WSP, which include hot water extraction (HWE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE). As a result, four WSP samples, WSPH, WSPU, WSPM and WSPE were acquired. Then, the difference of four WSP samples in yield, characterization and antioxidant activities in vitro were further compared. Experimental results showed that the four WSP samples had the same monosaccharide composition, but mere difference in the content; they all had typical IR spectra characteristic of polysaccharides. WSPU contained the highest contents of uronic acid and sulfate. The yield of WSPU was the highest and its antioxidant activity was the best. These results suggested that ultrasonic-assisted extraction was the best extraction method for WSP.