Sulfated heterorhamnans from the green seaweed Gayralia oxysperma: partial depolymerization, chemical structure and antitumor activity. (6th March 2015)

• Record Type: Journal Article
• Authors: Ropellato, Juliana; Carvalho, Mariana M.; Ferreira, Luciana G.; Noseda, Miguel D.; Zuconelli, Cristiane R.; Gonçalves, Alan G.; Ducatti, Diogo R.B.; Kenski, Juliana C.N.; Nasato, Pauline. L.; Winnischofer, Sheila M.B.; Duarte, Maria E.R.
• Is Part Of: Carbohydrate polymers. Volume 117(2015)
• Page Start: 476
• Page End: 485
• Publication Date: 2015-03-06
• Subjects: Sulfated polysaccharides -- Partial depolymerization -- Chemical structure -- Antitumor activity -- Gayralia oxysperma -- Green seaweed; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2014.09.089
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: Smith-degraded polysaccharides were obtained from sulfated heterorhamnans. The homogeneous fractions are highly sulfated and present M w of 109 and 251 kDa. Rhamnose residues are principally 3- and 2-linked: mono-, di-, and unsulfated. All polysaccharidic fractions were cytotoxic for human glioblastoma U87MG cells. Sulfated polysaccharides increased the number of cells in G1 phase via p53/p21. Abstract: Sulfated heterorhamnans produced by Gayralia oxysperma were utilized for the preparation of two homogeneous and highly sulfated Smith-degraded products ( M w of 109 and 251 kDa), which were constituted principally by 3-linked α-l -rhamnosyl units 2- or 4-sulfate and 2-linked α-l -rhamnosyl units 4- or 3, 4-sulfate, in different percentages. The homogeneous products and the crude extracts containing the sulfated heterorhamnans showed cytotoxic effect against U87MG cells. These sulfated polysaccharides induced an increase in the number of cells in G1 phase with concomitant increase of the mRNA levels of p53 and p21. The presence of 2-linked disulfated rhamnose residues together with the molecular weight could be important factors to be correlated with the inhibitory effect on human glioblastoma cells.