Biomolecular interactions control the shape of stains from drying droplets of complex fluids. (1st December 2015)

• Record Type: Journal Article
• Authors: Hurth, Cedric; Bhardwaj, Rajneesh; Andalib, Sahar; Frankiewicz, Christophe; Dobos, Andrew; Attinger, Daniel; Zenhausern, Frederic
• Is Part Of: Chemical engineering science. Volume 137(2015)
• Page Start: 398
• Page End: 403
• Publication Date: 2015-12-01
• Subjects: Biotin-streptavidin -- Microdroplets -- Polystyrene beads -- Biosensors -- Drying mechanism; Chemical engineering -- Periodicals; Génie chimique -- Périodiques; Chemical engineering; Periodicals; Electronic journals; 660
• DOI: 10.1016/j.ces.2015.06.059
• Languages: English
• ISSNs: 0009-2509

Abstract

Abstract: When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid–solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet–surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection. Highlights: Streptavidin-biotin model chosen for its wide variety of bio-applications. Direct influence of a biomolecular interaction on drying submicroliter droplets. Two different experimental manifestations are exposed.