Polystyrene micro and nano-particles induce metabolic rewiring in normal human colon cells: A risk factor for human health. (September 2022)

• Record Type: Journal Article
• Authors: Bonanomi, Marcella; Salmistraro, Noemi; Porro, Danilo; Pinsino, Annalisa; Colangelo, Anna Maria; Gaglio, Daniela
• Is Part Of: Chemosphere. Volume 303:Part 1(2022)
• Publication Date: 2022-09
• Subjects: Plastic pollution -- Metabolomics -- Risk factor; 1P-3H-5C 1-Pyrroline-5-carboxylate -- 3 PG 3-Phosphoglycerate -- 4-H-Glu 4-Hydroxyglutamate semialdehyde -- 6PGLac 6-Phosphonoglucono-d-Lactone -- AdeSucc Adenylsuccinic acid -- Ado Adenosine -- ADP Adenosine diphosphate -- AKG α-ketoglutarate -- AlloCys Allocystathionine -- AmSug Metab Amino-Sugar metabolism -- AOM Azoxymethane -- Asp Aspartic acid -- CCK-8 Cell Counting Kit-8 -- Cit Citric acid -- CYT D Cytochalasin D -- DYN Dynole 34-2 -- ECAR Extracellular Acidification Rate -- F1, 6BP Fructose 1, 6-bisphosphate -- F6P Fructose 6-phosphate -- Fum Fumaric acid -- G3P Glyceraldehyde 3-phosphate -- G6P Glucose 6-phosphate -- GDP-Glc GDP-glucose -- GEN Genistein -- Glc Glucose -- Glc-Ala Cycle Glucose-Alanine Cycle -- Gln Glutamine -- Glu 5-P Glutamic acid 5-phosphate -- Glu Metab Glutamate Metabolism -- Glu Glutamic acid -- Glyc 3-P Glycerol 3-phosphate -- Glyc Glycolysis -- GNG Gluconeogenesis -- GSH Metab Glutathione Metabolism -- GSH Reduced glutathione -- GSSG Oxidized Glutathione -- GTP Guanosine triphosphate -- IsoCit Isocitric acid -- Lac Lactic acid -- Mal Malic acid -- Mal-Asp Shuttle Malate-Aspartate Shuttle -- MP(s) Microplastic(s) -- NH3 Recyc Ammonia Recycling -- NP(s) Nanoplastic(s) -- OAA Oxaloacetic acid -- OCR Oxygen Consumption Rate -- OXPHOS Mitochondrial Oxidative Phosphorylation System -- PIT Pitstop 2–100 -- PMA Phorbol 12-myristate 13-acetate -- Ppi Pyrophosphate -- Pro Proline -- Pur Metab Purine metabolism -- Pyr Pyruvic acid -- PyrGlu Pyroglutamic acid -- Ribulose 5P Ribulose 5-phosphate -- ROS Reactive Oxygen Species -- Sedo 7P Sedoheptulose 7-phosphate -- Ser Serine -- Succ Succinic acid -- SuccSA Succinic acid semialdehyde -- TCA Cycle Tricarboxylic acid Cycle -- UAC Uric acid -- UDP-Glc UDP-Glucose -- UDP-GlcNAc Uridine diphosphate-N-acetylglucosamine -- γGC γ-Glutamylcysteine; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.134947
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Polystyrene is a thermoplastic polymer widely used in commercial products. Like all plastics, polystyrene can be degraded into microplastic and nanoplastic particles and ingested via food chain contamination. Although the ecological impact due to plastic contamination is well known, there are no studies indicating a carcinogenic potential of polystyrene microplastics (MPs) and nanoplastics (NPs). Here, we evaluated the effects of the MPs and NPs on normal human intestinal CCD-18Co cells. Our results show that internalization of NPs and MPs induces metabolic changes under both acute and chronic exposure by inducing oxidative stress, increasing glycolysis via lactate to sustain energy metabolism and glutamine metabolism to sustain anabolic processes. We also show that this decoupling of nutrients mirrors the effect of the potent carcinogenic agent azoxymethane and HCT15 colon cancer cells, carrying out the typical strategy of cancer cells to optimize nutrients utilization and allowing metabolic adaptation to environmental stress conditions. Taken together our data provide new evidence that chronic NPs and MPs exposure could act as cancer risk factor for human health. Graphical abstract: Image 1 Highlights: Acute and chronic NPs and MPs exposure cause metabolic rewiring and oxidative stress. Chronic NPs or MPs exposure induces decoupling of glucose and glutamine. NPs and MPs exposure acts as a cancer risk factor for human health.