Effects of tebuconazole application at different growth stages on rice grain quality of rice-based untargeted metabolomics. (September 2022)

• Record Type: Journal Article
• Authors: Li, Haocong; Li, Yong; Song, Lixiao; Cheng, Jinjin; Ge, Jing; Yu, Xiangyang; Sun, Wenjing
• Is Part Of: Chemosphere. Volume 303:Part 1(2022)
• Publication Date: 2022-09
• Subjects: Nontargeted metabolomics -- Tebuconazole -- White rice quality -- Protein -- Amylose; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2022.134920
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Tebuconazole (TEB) is a pesticide widely used in crops and has a strong control effect on fungal pathogens. TEB abuse has caused many food safety problems. In this study, the TEB residue in rice and the effect of TEB on white rice quality were investigated. The results showed that under two spraying concentrations, the TEB residue in rice was 11.21–19.05 μg/kg and 24.45–31.12 μg/kg, and there was no food safety risk of pesticide residue. When applying TEB according to the instructions, no significant effect was found. However, when 3 times the recommended TEB concentration was used at the filling stage, the protein content of white rice decreased significantly from 106.52 mg/g to 80.72 mg/g. At the jointing，heading and filling stage, the amylose content of white rice decreased to 53.95 mg/g, 48.77 mg/g and 49.04 mg/g from the blank control group. Plant metabolic analysis using LC-QTOF/MS revealed that the amino acid-related metabolic pathways in white rice were significantly affected by TEB. This is closely related to the decrease in protein accumulation in white rice and the stress response of rice plants. The increase in pantothenic acid content in white rice indicated that the glycolysis pathway of white rice plants was affected, and the consumption of starch and sucrose increased, leading to the inhibition of amylose accumulation in white rice. The increase in soluble sugar content and decrease in phosphocholine content in white rice suggested that rice plants were affected by TEB exposure, which produced similar effects under drought stress. Graphical abstract: Image 1 Highlights: Excessive use of tebuconazole (TEB) can affect the quality of rice grains. The effect of TEB on rice quality was related to the spraying time of rice growth. TEB can affect the amino acid metabolism of rice grains and thus affect the accumulation of rice grain total protein. TEB can affect glucose metabolism in rice and reduce amylose accumulation in rice grains.