A regular fucan sulfate from Stichopus herrmanni and its peroxide depolymerization: Structure and anticoagulant activity. (15th March 2021)

• Record Type: Journal Article
• Authors: Li, Xiaomei; Li, Shanni; Liu, Jie; Lin, Lisha; Sun, Huifang; Yang, Wenjiao; Cai, Ying; Gao, Na; Zhou, Lutan; Qin, Hongbo; Yin, Ronghua; Zhao, Jinhua
• Is Part Of: Carbohydrate polymers. Volume 256(2021)
• Publication Date: 2021-03-15
• Subjects: FS fucan sulfate -- dFS depolymerized fucan sulfate -- AT antithrombin -- HCII heparin cofactor II -- IIa thrombin -- ShFS FS from Stichopus herrmanni -- Mw weight-average molecular mass -- DS dermatan sulfate -- HPGPC high-performance gel permeation chromatography -- RID differential refractive index detector -- DAD diode array detector -- MALLS multi-angle laser light-scattering -- Fuc α-L-fucose -- Fuc2S 2-mono-O-sulfated fucose -- GPC gel permeation chromatography -- APTT activated partial thromboplastin time -- PT prothrombin time -- TT thrombin time -- LMWH low molecular weight heparin; fucan sulfate -- peroxide depolymerization -- oligosaccharides -- anti-thrombin activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2020.117513
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: A fucan sulfate with a regular structure was obtained from Stichopus herrmanni . Structure of the fucan sulfate was clarified. A possible process of peroxide depolymerization of this fucan sulfate was proposed. Molecular weight and anti-thrombin activity relationships were discussed. Abstract: Marine sulfated polysaccharides have aroused widespread concern for their various structures and bioactivities. Peroxide depolymerization is a common strategy in analysis of structures and structure-activity relationships of polysaccharides. However, confirming the depolymerization process and exact structures of the degradation products is still a considerable challenge. This study reported the structures of a fucan sulfate (FS) from sea cucumber Stichopus herrmanni and its depolymerized products (dFS) prepared by peroxide degradation. The FS was elucidated with a highly regular structure, {-3)- L -Fuc2S -(α1-}n . Structure analysis of oligosaccharides purified from dFS suggested that peroxide degradation involved in cleavage of glycosidic bonds and oxidative modification of reducing end of sugar residue, while no break in sugar ring was observed. Both FS and series of dFSs exhibited significant anticoagulant activities due to their anti-thrombin effects in presence of heparin cofactor II and their potencies were related to their molecular sizes, dFS with ∼ 20 kDa showed the strongest activity.