Genome-wide association mapping identifies an SNF4 ortholog that impacts biomass and sugar yield in sorghum and sugarcane. (15th March 2022)

• Record Type: Journal Article
• Authors: Upadhyaya, Hari D; Wang, Lihua; Prakash, Chudamani Sharma; Liu, Yanlong; Gao, Li; Meng, Ruirui; Seetharam, Kaliyamoorthy; Gowda, C L Laxmipathi; Ganesamurthy, Kulandaivelu; Singh, Shailesh Kumar; Kumar, Rajendra; Li, Jieqin; Wang, Yi-Hong
• Editors: Rebetzke, Greg
• Is Part Of: Journal of experimental botany. Volume 73:Number 11(2022)
• Page Start: 3584
• Page End: 3596
• Publication Date: 2022-03-15
• Subjects: Association mapping -- juice yield -- resequencing -- sorghum -- sugar yield -- sugarcane -- sugar content -- SNF4; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erac110
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: Sorghum is a feed/industrial crop in developed countries and a staple food elsewhere in the world. This study evaluated the sorghum mini core collection for days to 50% flowering (DF), biomass, plant height (PH), soluble solid content (SSC), and juice weight (JW), and the sorghum reference set for DF and PH, in 7–12 testing environments. We also performed genome-wide association mapping with 6 094 317 and 265 500 single nucleotide polymorphism markers in the mini core collection and the reference set, respectively. In the mini core panel we identified three quantitative trait loci for DF, two for JW, one for PH, and one for biomass. In the reference set panel we identified another quantitative trait locus for PH on chromosome 6 that was also associated with biomass, DF, JW, and SSC in the mini core panel. Transgenic studies of three genes selected from the locus revealed that Sobic.006G061100 ( SbSNF4-2 ) increased biomass, SSC, JW, and PH when overexpressed in both sorghum and sugarcane, and delayed flowering in transgenic sorghum. SbSNF4-2 encodes a γ subunit of the evolutionarily conserved AMPK/SNF1/SnRK1 heterotrimeric complexes. SbSNF4-2 and its orthologs will be valuable in genetic enhancement of biomass and sugar yield in plants. Abstract : Flowering time, biomass, plant height, soluble solid content, and juice weight in sorghum are controlled by the gene SbSNF4-2, encoding an ortholog of the γ subunit of AMPK/SNF1/SnRK1 complexes.