Self‐Regeneration and Self‐Healing in DNA Origami Nanostructures. Issue 9 (28th January 2021)

• Record Type: Journal Article
• Authors: Scheckenbach, Michael; Schubert, Tom; Forthmann, Carsten; Glembockyte, Viktorija; Tinnefeld, Philip
• Is Part Of: Angewandte Chemie international edition. Volume 60:Issue 9(2021)
• Page Start: 4931
• Page End: 4938
• Publication Date: 2021-01-28
• Subjects: DNA -- molecular devices -- nanotechnology -- self-assembly -- self-healing; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202012986
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: DNA nanotechnology and advances in the DNA origami technique have enabled facile design and synthesis of complex and functional nanostructures. Molecular devices are, however, prone to rapid functional and structural degradation due to the high proportion of surface atoms at the nanoscale and due to complex working environments. Besides stabilizing mechanisms, approaches for the self‐repair of functional molecular devices are desirable. Here we exploit the self‐assembly and reconfigurability of DNA origami nanostructures to induce the self‐repair of defects of photoinduced and enzymatic damage. We provide examples of repair in DNA nanostructures showing the difference between unspecific self‐regeneration and damage specific self‐healing mechanisms. Using DNA origami nanorulers studied by atomic force and superresolution DNA PAINT microscopy, quantitative preservation of fluorescence properties is demonstrated with direct potential for improving nanoscale calibration samples. Abstract : Self‐assembly and reconfigurability of DNA origami is exploited to realize self‐repairing functional nanodevices. A self‐regenerating brightness label is realized by constant exchange of labeling strands from solution. Structural self‐healing of a DNA origami nanoruler is achieved by excess of staple strands that exchange with enzymatically or photo‐induced damaged strands.