Life-Long Neural Stem Cells Are Fate-Specified at an Early Developmental Stage. (6th August 2020)

• Record Type: Journal Article
• Authors: Tanaka, Aoi; Ishida, Shohei; Fuchigami, Takahiro; Hayashi, Yoshitaka; Kuroda, Anri; Ikenaka, Kazuhiro; Fukazawa, Yugo; Hitoshi, Seiji
• Is Part Of: Cerebral cortex. Volume 30:Number 12(2020)
• Page Start: 6415
• Page End: 6425
• Publication Date: 2020-08-06
• Subjects: barcoding -- clonal analysis -- lentivirus -- mouse neural stem cells -- neurospheres; Cerebral cortex -- Periodicals; Brain -- Periodicals; 612.825
• DOI: 10.1093/cercor/bhaa200
• Languages: English
• ISSNs: 1047-3211

Abstract

Abstract: The origin and life-long fate of quiescent neural stem cells (NSCs) in the adult mammalian brain remain largely unknown. A few neural precursor cells in the embryonic brain elongate their cell cycle time and subsequently become quiescent postnatally, suggesting the possibility that life-long NSCs are selected at an early embryonic stage. Here, we utilized a GFP-expressing lentivirus to investigate the fate of progeny from individual lentivirus-infected NSCs by identifying the lentiviral integration site. Our data suggest that NSCs become specified to two or more lineages prior to embryonic day 13.5 in mice: one NSC lineage produces cells only for the cortex and another provides neurons to the olfactory bulb. The majority of neurosphere-forming NSCs in the adult brain are relatively dormant and generate very few cells, if any, in the olfactory bulb or cortex, and this NSC population could serve as a reservoir that is occasionally reactivated later in life.