A missense mutation in WRKY32 converts its function from a positive regulator to a repressor of photomorphogenesis. Issue 1 (7th January 2022)

• Record Type: Journal Article
• Authors: Zhou, Hua; Zhu, Wei; Wang, Xuncheng; Bian, Yeting; Jiang, Yan; Li, Jian; Wang, Lixia; Yin, Ping; Deng, Xing Wang; Xu, Dongqing
• Is Part Of: New phytologist. Volume 235:Issue 1(2022)
• Page Start: 111
• Page End: 125
• Publication Date: 2022-01-07
• Subjects: Arabidopsis -- COP1 -- HY5 -- light signaling -- photomorphogenesis -- WRKY32; Botany -- Periodicals; 580
• DOI: 10.1111/nph.17932
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) mediates various cellular and physiological processes in plants by targeting a large number of substrates for ubiquitination and degradation. In this study, we reveal that a substitution of Pro for Leu at amino acid position 409 in WRKY32 largely suppresses the short hypocotyls and expanded cotyledon phenotypes of cop1‐6 . WRKY32 P409L promotes hypocotyl growth and inhibits the opening of cotyledons in Arabidopsis . Loss of WRKY32 function mutant seedlings display elongated hypocotyls, whereas overexpression of WRKY32 leads to shortened hypocotyls. WRKY32 directly associates with the promoter regions of HY5 to activate its transcription. COP1 interacts with and targets WRKY32 for ubiquitination and degradation in darkness. WRKY32 P409L exhibits enhanced DNA binding ability and affects the expression of more genes compared with WRKY32 in Arabidopsis . Our results not only reveal the basic role for WRKY32 in promoting photomorphogenesis, but also provide insights into manipulating plant growth by engineering key components of light signaling. Abstract : See also the Commentary on this article by Demarsy, 235 : 5–7 .