A ratiometric fluorescence strategy based on copper nanoclusters/carbon dots for sensitive detection of doxorubicin. (28th March 2022)

• Record Type: Journal Article
• Authors: Li, Feifei; Fan, Pengfei; Chen, Xinbei; Lin, Xi; Liu, Can; Hu, Congcong; Yang, Shengyuan; Xiao, Fubing
• Is Part Of: Luminescence. Volume 37:Number 6(2022)
• Page Start: 868
• Page End: 875
• Publication Date: 2022-03-28
• Subjects: carbon dots -- copper nanoclusters -- doxorubicin -- IFE; Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.4230
• Languages: English
• ISSNs: 1522-7235

Abstract

Abstract: Sensitive detection of doxorubicin (DOX) is critical for clinical theranostics. A novel ratiometric fluorescence strategy based on the inner filter effect (IFE) has been established for the sensitive detection of DOX by designing a ratiometric fluorescence probe. In the presence of DOX, the fluorescence intensity of copper nanoclusters (CuNCs) at 485 nm decreases, and the fluorescence intensity of carbon dots at 560 nm increases. Therefore, DOX can be quantitatively detected by measuring the ratio of the fluorescence intensities at 560 and 485 nm ( F 560 / F 485 ). The F 560 / F 485 ratio exhibits a linear correlation with the DOX concentration in the range from 1.0 × 10 −8 M to 1.0 × 10 −4 M with the detection limit of 3.7 nM. Furthermore, this method was also successfully applied to the analysis of DOX in human plasma samples, affording an effective platform for drug safety management. Abstract : The CuNCs/CDs fluorescent probe produces two separate fluorescence emission peaks at 485 and 560 nm at the excitation wavelength of 430 nm. In the presence of DOX, the fluorescence intensity of CuNCs ( F 485) decreases, while the fluorescence intensity of CDs ( F 560) increases, resulting in a change in the fluorescence intensity ratio ( F 560/ F 485). Therefore, DOX can be quantitatively detected with high sensitivity by measuring the ratio of F 560/ F 485.