Synthesis, DNA-binding abilities, and in vitro antitumor activity of water-soluble copper porphyrin and its Schiff-base complexes. (22nd March 2022)

• Record Type: Journal Article
• Authors: Hasi, Qi-Meige; Guo, Yuping; Yang, Jiaqi; Mu, Xiaotong; Chen, Lihua; Wang, Shanshan; Xiao, Chaohu; Zhang, Yuhan; Han, Zhichao
• Is Part Of: New journal of chemistry. Volume 46:Number 14(2022)
• Page Start: 6811
• Page End: 6818
• Publication Date: 2022-03-22
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/d2nj00326k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Three new water-soluble porphyrin Cu(ii )-complexes (CuP-1, CuP-2, and CuP-3 ) were prepared and characterized, which had the ability to bind ct-DNA and good cytotoxicity. CuP-1 showed the best antiproliferative activity towards TCA8113 cells. Abstract : Herein, three new water-soluble porphyrin Cu(ii )-complexes (denoted as CuP-1, CuP-2, and CuP-3, respectively) have been successfully prepared and well characterized. A variety of spectral analysis techniques showed that the three complexes bind with calf thymus DNA (ct-DNA) via an intercalation mode, and CuP-1 showed the strongest binding affinity with ct-DNA. In addition, the effects of the water-soluble porphyrin Cu(ii )-complexes (CuP-1, CuP-2, and CuP-3 ) on the survival rates of different tumor cells were tested via MTT assays. The results showed that all the complexes exhibit high cytotoxicity towards three tumor cell types, especially CuP-1, which showed strong cytotoxicity towards TCA8113 tumor cells. In addition, the cell cycle distribution and induction of apoptosis were studied via flow cytometric analysis based on three tumor cell lines, and the results demonstrated that the tested complexes may induce cell death through early apoptosis and inducing a block on the G1 /S phase of the cell cycle.