Preparation of chiral aryl alcohols: a controllable enzymatic strategy via light-driven NAD(P)H regeneration. (14th March 2022) - Xing, Xiu
Abstract : Controllable and mild photoenzymatic production of chiral alcohols was realized by coupling a photochemical NAD(P)H regeneration system with ( R )- or ( S )-selective ketoreductases. Abstract : Controllable and mild photoenzymatic production of chiral alcohols was realized by coupling a versatile photochemical NAD(P)H regeneration system with ( R )- or ( S )-selective ketoreductases. The efficiency of NAD(P)H regeneration was improved using a Rhodium functionalized metal organic framework, namely Rh-UiO-67, to adjust and control electron transport and electron utilization. Furthermore, six different ketoreductases could be successfully immobilized on Rh-UiO-67 and combined with the light-driven NAD(P)H regeneration system to produce chiral aryl alcohols. Various chiral alcohols with complementary ( R )- and ( S )-conformations can be constructed by this method with high yields (97%) and excellent stereoselectivity (>99% ee).