New insights into neutrophil and Leishmania infantum in vitro immune interactions. (June 2015)

• Record Type: Journal Article
• Authors: Marques, Cláudia S.; Passero, Luiz Felipe D.; Vale-Gato, Inês; Rodrigues, Armanda; Rodrigues, Olivia Roos; Martins, Catarina; Correia, Ivone; Tomás, Ana M.; Alexandre-Pires, Graça; Ferronha, M. Helena; Santos-Gomes, Gabriela M.
• Is Part Of: Comparative immunology, microbiology and infectious diseases. Volume 40(2015)
• Page Start: 19
• Page End: 29
• Publication Date: 2015-06
• Subjects: CatG cathepsin G -- FCS forward light scatter -- FITC fluorescein isothiocyanate -- fMLP N-formyl-Met-Leu-Phe -- GFP green fluorescent protein -- HPRT hypoxanthine guanine phosphoribosyl transferase -- IL interleukin -- Mϕ macrophages -- CCL3/MIP-1 macrophage inflammatory protein -- CXCL1/KC keratinocyte chemoattractant -- CCL2/MCP macrophage chemotactic protein -- MPO myeloperoxidase -- NBT nitroblue tetrazolium -- NE neutrophil elastase -- NET neutrophil extracellular trap -- PI propidium iodide -- PMN polymorphonuclear cells -- SSC side light scatter -- TLR toll-like receptor -- TNF tumour necrosis factor; Neutrophils -- Zoonotic visceral leishmaniasis -- Leishmania infantum -- C57BL/6 mice -- Phagocytosis -- Chemotaxis -- Granule exocytosis -- Apoptosis -- Gene expression; Communicable diseases in animals -- Periodicals; Veterinary immunology -- Periodicals; Veterinary microbiology -- Periodicals; Immunology -- Periodicals; Microbiology -- Periodicals; Communicable diseases -- Periodicals; Communicable Diseases -- immunology -- Periodicals; Communicable Diseases -- veterinary -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Veterinary Medicine -- Periodicals; Immunologie -- Périodiques; Microbiologie -- Périodiques; Maladies infectieuses -- Périodiques; Communicable diseases; Immunology; Microbiology; Electronic journals; Periodicals; 636.08969
• DOI: 10.1016/j.cimid.2015.03.003
• Languages: English
• ISSNs: 0147-9571

Abstract

Abstract: The interaction between polymorphonuclear leukocytes (PMN) or neutrophils and Leishmania became an interesting focus of research, since PMN turn out to be essential cells in transiently hosting the parasites. This study aims to evaluate whether L. infantum, the etiological agent of zoonotic visceral leishmaniasis, influences the in vitro functional activity of murine neutrophils. Phagocytosis, chemotaxis, oxidative burst, degranulation and apoptosis assays were performed. Cytokines, chemokines and toll-like receptors gene expression were evaluated by Real-time PCR. Results indicate that some of the innate features of PMN immunity were activated when in contact with L. infantum. However, parasites might negatively interfere with PMN defense mechanisms compromising the link between innate and acquired immunity. This work provides additional insights on the inflammatory immune interactions between neutrophils and L. infantum highlighting the role of PMN in Leishmania infection.