Recovery of cellulose and lignin from Eucalyptus by-product and assessment of cellulose enzymatic hydrolysis. (June 2022)

• Record Type: Journal Article
• Authors: Roberto Paz Cedeno, Fernando; Belon de Siqueira, Breno; Gabriel Solorzano Chavez, Eddyn; Ulises Miranda Roldán, Ismael; Moreira Ropelato, Leonardo; Paul Martínez Galán, Julián; Masarin, Fernando
• Is Part Of: Renewable energy. Volume 193(2022)
• Page Start: 807
• Page End: 820
• Publication Date: 2022-06
• Subjects: Eucalyptus by-product -- Pretreatment -- Cellulose -- Lignin -- Enzymatic hydrolysis -- Cello-oligosaccharides -- Glucose; Renewable energy sources -- Periodicals; Power resources -- Periodicals; Énergies renouvelables -- Périodiques; Ressources énergétiques -- Périodiques; 333.794
• DOI: 10.1016/j.renene.2022.05.027
• Languages: English
• ISSNs: 0960-1481

Abstract

Abstract: In this study, a Eucalyptus by-product was fractionated into cellulose and lignin by pretreatment with NaClO2 to obtain α-cellulose, and hydrothermal pretreatment followed by sulfite-NaOH to obtain cellulose and lignin. The recovered celluloses were chemically and structurally characterized and enzymatically hydrolyzed using commercial enzyme preparations. The α-cellulose and cellulose treated with sulfite-NaOH had cellulose contents of 70.1% and 61.1%, respectively. α-cellulose hydrolyzed with Celluclast showed potential for cello-oligosaccharides formation, resulting in a maximum conversion of 30%, while Cellic CTec 2 resulted in 100% conversion to glucose, whereas cellulose treated with sulfite-NaOH showed potential for conversion to glucose with Cellulcast (44%) and Cellic CTec 2 (78%). The liquor from the sulfite-NaOH process resulted in a 20.8% yield of sulfonated lignin recovery. The use of different pretreatment techniques for the Eucalyptus by-product can be alternatives for the generation of products with high added value and commercial potential, such as cello-oligosaccharides, glucose, and sulfonated lignin. Graphical abstract: Image 1 Highlights: Eucalyptus by-product treated with NaClO2 was hydrolyzed to cello-oligosaccharides. Eucalyptus by-product thermotreated and treated with sulfite-NaOH was hydrolyzed to glucose. Glucose hydrolysate has potential for 2G ethanol production. Sulphonated lignin was recovered from sulfite-NaOH hydrolysis liquor. Sulphonated lignin can be used for energy cogeneration.