Bisdemethoxycurcumin inhibits oxidative stress and antagonizes Alzheimer's disease by up‐regulating SIRT1. Issue 7 (22nd May 2020)

• Record Type: Journal Article
• Authors: Xu, Yan; Hu, Rong; He, Duanqun; Zhou, Guijuan; Wu, Heng; Xu, Chenlin; He, Bing; Wu, Lin; Wang, Yilin; Chang, Yunqian; Ma, Rundong; Xie, Ming; Xiao, Zijian
• Is Part Of: Brain and behavior. Volume 10:Issue 7(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-05-22
• Subjects: Alzheimer's disease -- bisdemethoxycurcumin -- oxidative stress -- silent information regulator 1; Neurology -- Periodicals; Neurosciences -- Periodicals; Psychology -- Periodicals; Psychiatry -- Periodicals; 616.8005
• DOI: 10.1002/brb3.1655
• Languages: English
• ISSNs: 2162-3279

Abstract

Abstract: Introduction: Alzheimer's disease (AD) is a progressive neurodegenerative disease. It can lead to progressive cognitive impairment, memory loss, and behavioral alterations. So far, the exact cellular and molecular mechanisms underlying this disorder remain unclear. And there are no effective treatments to prevent, halt, or reverse AD. In recent years, Chinese traditional medicine has become a new force in the treatment of AD, and the typical representatives of natural herbal ingredients are curcumin and its derivatives. Bisdemethoxycurcumin (BDMC), which is a classical derivative of curcumin, was found to have neuroprotective effects against a cell model of Alzheimer's disease (AD) in our previous studies. This study investigated the intrinsic mechanism of BDMC against AD in animal models. Methods: In this study, BDMC was injected into the lateral ventricles of normal C57BL/6 mice, APP/PS mice, and APP/PS mice treated with EX527 (the inhibitor of SIRT1). Y maze and Morris water maze were used to test the learning and memory ability of mice. Nissl staining was used to observe the morphological changes of neurons. Immunofluorescence staining was used to detect Aβ deposition in mice. The activities of GSH and SOD were determined to observe the levels of oxidative stress in mice. And Western blot analyses were used to detect content of SIRT1 in mice. Results: In the APP/PS mice, after BDMC intervention, their cognitive function improved, oxidative stress adjusted, the number of neurons increased, Aβ deposition decreased, and the level of SIRT1 expression increased. However, when SIRT1 is inhibited, BDMC on the improvement in the learning and memory ability and the improvement on oxidative stress in APP/PS1 mice were reversed. Conclusion: Our findings demonstrated that in the AD mice, BDMC has antagonistic effect on AD. And an intermediate step in the antagonism effect is caused by SIRT1 upregulation, which leading to decreased oxidative stress. Based on these, we concluded that BDMC injection into the lateral ventricle can act against AD by upregulating SIRT1 to antioxidative stress. Abstract : In this work, we observed that the level of cognitive function in APP/PS1 mice is decreased and the morphology of hippocampal neurons is markedly disrupted; however, the level of cognitive function and the pathological morphology of the hippocampus in APP/PS1 mice with lateral ventricle injection of BDMC are improved. And we also found that BDMC could not antagonize AD after SIRT1 was inhibited. It provides another new direction for the development of drugs for Alzheimer's disease.