Synthesis and Functional Reconstitution of Light‐Harvesting Complex II into Polymeric Membrane Architectures. Issue 49 (16th October 2015)

• Record Type: Journal Article
• Authors: Zapf, Thomas; Tan, Cherng‐Wen Darren; Reinelt, Tobias; Huber, Christoph; Shaohua, Ding; Geifman‐Shochat, Susana; Paulsen, Harald; Sinner, Eva‐Kathrin
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 49(2015)
• Page Start: 14664
• Page End: 14668
• Publication Date: 2015-10-16
• Subjects: light‐harvesting complexes -- membrane proteins -- membrane models -- protein expression -- synthetic polymer membranes; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201506304
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: One of most important processes in nature is the harvesting and dissipation of solar energy with the help of light‐harvesting complex II (LHCII). This protein, along with its associated pigments, is the main solar‐energy collector in higher plants. We aimed to generate stable, highly controllable, and sustainable polymer‐based membrane systems containing LHCII–pigment complexes ready for light harvesting. LHCII was produced by cell‐free protein synthesis based on wheat‐germ extract, and the successful integration of LHCII and its pigments into different membrane architectures was monitored. The unidirectionality of LHCII insertion was investigated by protease digestion assays. Fluorescence measurements indicated chlorophyll integration in the presence of LHCII in spherical as well as planar bilayer architectures. Surface plasmon enhanced fluorescence spectroscopy (SPFS) was used to reveal energy transfer from chlorophyll b to chlorophyll a, which indicates native folding of the LHCII proteins. Abstract : Designed to catch the light : Light‐harvesting complex II (LHCII) is one of the most abundant and best studied membrane proteins in the world. The integration of LHCII into polymeric membranes through cell‐free protein synthesis offers a means to overcome random insertion and other technological limitations of lipid or detergent‐based arrays for LHC‐based light harvesting.