Characterisation of blaKPC-2–harbouring plasmids recovered from Pseudomonas aeruginosa ST654 and ST235 high-risk clones. (June 2022)

• Record Type: Journal Article
• Authors: Cejas, Daniela; Elena, Alan; González-Espinosa, Francisco E; Pallecchi, Lucia; Vay, Carlos; Rossolini, Gian Maria; Gutkind, Gabriel; Di Pilato, Vincenzo; Radice, Marcela
• Is Part Of: Journal of global antimicrobial resistance. Volume 29(2022)
• Page Start: 310
• Page End: 312
• Publication Date: 2022-06
• Subjects: Pseudomonas aeruginosa -- ST235 -- ST654 -- blaKPC-2 -- Plasmids; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2022.04.017
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: KPC-2 producing Pseudomonas aeruginosa high-risk clones ST235 and ST654 Non conjugative plasmids harbouring bla KPC-2 bla KPC-2 was located in Tn 4401b in P. aeruginosa ST654 bla KPC-2 was located in a novel architecture in P. aeruginosa ST235 ABSTRACT: Objective: The main objectives were to describe two bla KPC-2 plasmids recovered from Pseudomonas aeruginosa isolates belonging to the ST654 and ST235 high-risk clones, and to compare with complete sequences of bla KPC-2 harbouring plasmids available in public databases. Methods: Antimicrobial susceptibility was determined according to CLSI (Clinical and Laboratory Standards Institute) guidelines. Genomes were sequenced using an Illumina MiSeq platform, and bla KPC-2 plasmid sequences were achieved using MinION platform. Sequences were analysed using Unicycler and RAST. In silico predictions of the isolates sequence type (ST), antimicrobial resistance genes, plasmid replicon typing and MOB relaxases were fulfilled using bioinformatics tools. Results: PA_2047 and PA_HdC isolates corresponded to the high-risk clones ST654 and ST235, respectively. The carbapenem resistance was mediated by KPC-2. Both bla KPC-2 harbouring plasmids, pPA_2047 and pPA_HdC, were different among them, non-conjugative and untypable by PlasmidFinder. pPA_2047 presented high identity with a Pae-13 plasmid, and these both located bla KPC-2 in Tn 4401b isoform. pPA_HdC displayed a novel architecture, and the genetic context of bla KPC-2 was original. Besides the bla KPC-2 gene, resistance genes to aminoglycosides and quinolones were detected, including the novel phosphotransferase CrpP in PA_HdC. Conclusion: This study expands the limited knowledge about the molecular epidemiology of bla KPC-2 in P. aeruginosa from Latin America. Two novel plasmids harbouring bla KPC-2 were described that were untypable by their incompatibility group. The plasmid recovered from P. aeruginosa PA_HdC (ST235) displayed a novel architecture and an original context for bla KPC-2 . On the other hand, the genetic platform carrying bla KPC-2 in P. aeruginosa PA_2047 (ST654) seems to a be a classical one.