Response relationship of environmental factors caused by toluene concentration during leaching of capillary zone. (1st September 2022) - Zuo, Rui
Abstract: Due to the leaching of capillary water, the petroleum pollutants initially trapped in vadose zone may migrate to lower aquifer, thus increasing the risk of groundwater pollution. In order to explore the effect of capillary leaching on toluene-contaminated soil and the relationship between toluene concentration (TC) and environmental factors (EFs) during the leaching process, the sterilized and non-sterilized soil column experiments were designed. The EFs were used to estimate TC. The results showed that the difference between leaching and volatilization rates directly determined the changing trend of toluene concentration in capillary water. The toluene concentration in the medium always showed decreasing trend due to leaching. The indigenous microbial community structure of the non-sterilized soil column was analyzed by 16S rRNA sequencing. It was found that indigenous microorganisms could degrade toluene after 33.0 days of acclimatation. The microbial population was dominated by bacteria, among them the Ellin6055 strain and Pseudomonas, Pseudoxanthomonas, Cupriavidus, Bdellovibrio, Sphingobium, Phenylobacterium, Ramlibacter, Bradyrhizobium, Shinella genera. The Pseudomonas was the most crucial bacterial genus that degraded toluene. Indigenous microbial degradation was the fundamental reason for strong response relationship. Furthermore, we suggested a relationship of function between environmental factors (pH, DO, ORP) and time (t) for toluene attenuation: C 0 + l n ( e A t α B γ C β ) = C T o l u e n e, ( α, β, γ represent the pH, DO, and ORP in leaching capillary water, respectively; A, B, and C represent undetermined coefficients), and the fitting coefficient R 2 > 0.950. This relationship can only characterize the attenuation process of capillary zone leaching on toluene. However, it may still be utilized to give a theoretical foundation for understanding the dynamic of pollutant concentration change processes under specific environmental factors. Graphical abstract: Image 1 Highlights: Leaching converts free phase of toluene into dissolved phase in capillary zone. Toluene attenuation during leaching is caused by volatilization and degradation. After 33 days of leaching, Pseudomonas is primary strain for toluene degradation. The relationship of environmental factors and time to concentration is C 0 + l n ( e A t α B γ C β ) = C T o l u e n e .