The innate immune stimulant Amplimune® is safe to administer to young feedlot cattle. Issue 6 (27th February 2022)

• Record Type: Journal Article
• Authors: Alexander, AL; Doyle, E; Ingham, AB; Colditz, I; McRae, G; Alkemade, S; Cervantes, MP; Hine, BC
• Is Part Of: Australian veterinary journal. Volume 100:Issue 6(2022)
• Page Start: 261
• Page End: 270
• Publication Date: 2022-02-27
• Subjects: Amplimune -- cattle -- respiratory disease -- trained immunity; Veterinary medicine -- Australia -- Periodicals; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; 636.089
• DOI: 10.1111/avj.13156
• Languages: English
• ISSNs: 0005-0423

Abstract

Abstract : Background: Infectious disease has a significant impact on livestock production. Availability of alternatives to antibiotics to prevent and treat disease is required to reduce reliance on antibiotics while not impacting animal welfare. Innate immune stimulants, such as mycobacterium cell wall fractions (MCWF), are used as alternatives to antibiotics for the treatment and prevention of infectious disease in a number of species including cattle, horses and dogs. This study aimed to evaluate the safety of Amplimune®, an MCWF‐based immune stimulant, for weaner Angus cattle. Methods: On day −1 and 0, sixty mixed‐sex Angus weaner cattle were transported for 6 h before being inducted and housed in a large single pen, simulating feedlot induction conditions. The cattle were assigned to one of six treatment groups (n = 10 per group): 2 mL Amplimune intramuscularly (2IM); 2 mL Amplimune subcutaneously (2SC); 5 mL Amplimune intramuscularly (5IM); 5 mL Amplimune subcutaneously (5SC); 5 mL saline intramuscularly (SalIM) and 5 mL saline subcutaneously (SalSC) on day 0 following transportation. Body temperature, body weight, concentrations of circulating pro‐inflammatory cytokines (TNFα, IL‐1β, IL‐6 and IL‐12) and haematology parameters were measured at various times up to 96 h post‐treatment. Results: No adverse effects from Amplimune treatment were observed. Amplimune induced an increase in circulating cytokine TNFα concentrations, total white blood cell count and lymphocyte counts indicative of activation of the innate immune system without causing an excessive inflammatory response. Conclusions: Results confirm that Amplimune can be safely administered to beef cattle at the dose rates and via the routes of administration investigated here.