Activin A attenuates apoptosis of granulosa cells in atretic follicles through ERβ‐induced autophagy. Issue 6 (7th March 2022) - Liu, Ning
Abstract: It is well known that approximately 99% of ovarian follicles in mammals suffer from a degenerative process known as atresia, which is a huge waste of genetic resource in female animals. Studies have shown that activin A (ACT‐A) is located in ovarian granulosa cells and has different effects in granulosa cell depending on species. Although granulosa cells play a critical role during follicular atresia, the mechanism of action of ACT‐A in bovine ovarian granulosa cells (BGC) is poorly understood. In this study, we first determined the apoptosis of BGCs isolated from growth follicles and atretic follicles respectively. Then, BGC isolated from atretic follicles were used as a model to elucidate the role of ACT‐A in cattle ovary. The results showed that apoptosis occurred in both growing follicles and atretic follicles, and the percentage of apoptotic cells in atretic follicles was higher than that in growing follicles. The current results indicated that ACT‐A can attenuate apoptosis of BGC by maintaining the function of BGC in atretic follicles. Increased ERβ induced by ACT‐A promoted BGC autophagy but had no effect on apoptosis. In summary, this study suggests that ACT‐A attenuates BGC apoptosis in atretic follicles by ERβ‐mediated autophagy signalling.