Direct and Simultaneous Identification of Multiple Mitochondrial Reactive Oxygen Species in Living Cells Using a SERS Borrowing Strategy. (21st April 2022)

• Record Type: Journal Article
• Authors: Lin, Shichao; Ze, Huajie; Zhang, Xia‐Guang; Zhang, Yue‐Jiao; Song, Juan; Zhang, Huimin; Zhong, Han‐Liang; Yang, Zhi‐Lan; Yang, Chaoyong; Li, Jian‐Feng; Zhu, Zhi
• Is Part Of: Angewandte Chemie. Volume 134:Number 25(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-04-21
• Subjects: Core–Shell Nanoprobe -- Living Cells -- Reactive Oxygen Species -- SERS Borrowing -- Spectroscopic Identification; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202203511
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Identification of different mitochondrial reactive oxygen species (ROS) simultaneously in living cells is vital for understanding the critical roles of different ROS in biological processes. To date, it remains a great challenge to develop ROS probes for direct and simultaneous identification of multiple ROS with high specificity. Herein, we report a SERS‐borrowing‐strategy‐based nanoprobe (Au@Pt core–shell nanoparticles) for simultaneous and direct identification of different ROS by their distinct Raman fingerprints. Isotope substitution experiments and DFT calculations confirmed the ability of Au@Pt nanoprobe to capture and identify different mitochondrial ROS (i.e. ⋅OOH, H2 O2, and ⋅OH). When functionalized with triphenylphosphine (TPP), the Au@Pt‐TPP nanoprobe located to mitochondria and detected multiple ROS simultaneously in living cells under oxidative stimulation. Our method offers a new tool for the study of the functions of various ROS in biological processes. Abstract : A SERS‐borrowing‐strategy‐based Au@Pt core–shell nanoprobe (Au@Pt NP) was developed for simultaneous and direct spectroscopic detection and identification of multiple reactive oxygen species (ROS). Triphenylphosphine‐functionalized Au@Pt‐TPP NPs were able to locate to the mitochondria for the detection and identification of different mitochondrial ROS simultaneously in living cells under oxidative stress.