Impact of the surface modifications and cell culture techniques on the biomechanical properties of PDMS in relation to cell growth behavior. Issue 12 (13th August 2022)

• Record Type: Journal Article
• Authors: Aydemir, Duygu; Dogru, Sedat; Alaca, B. Erdem; Ulusu, Nuriye Nuray
• Is Part Of: International journal of polymeric materials. Volume 71:Issue 12(2022)
• Page Start: 886
• Page End: 897
• Publication Date: 2022-08-13
• Subjects: Ethanol -- modulus of elasticity -- oxidative stress -- PDMS -- stress relaxation -- UV; Polymers -- Periodicals; Plastics -- Periodicals; 620.19205
• DOI: 10.1080/00914037.2021.1919670
• Languages: English
• ISSNs: 0091-4037

Abstract

Abstract: PDMS is the most widely used material in the microfluidic systems because of its advantageous properties including ease of manufacture, low cost, transparency, and gas permeability. Although all biomaterials need to be sterilized for usage for in vitro or in vivo systems to avoid possible contaminants, none of them proved effective in providing the gold standards so far for PDMS sterilization. Therefore we evaluated the influence of both UV and ethanol sterilization of PDMS substrates on the cell behavior via investigating cell proliferation, oxidative stress and nutrient metabolism of 293 T cells growing on the PDMS first time in the literature. Modulus of elasticity and stress relaxation of UV-treated PDMS samples increased compared to the ethanol-treated ones leading to a state of surface stiffness preferable for cell growth and proliferation. Oxidative stress metabolism of cells growing on the UV-treated PDMS was more stable compared to the ethanol-treated samples. Trace element and mineral metabolism are not impaired in the cells growing on the UV-treated PDMS samples. First time in the literature, we showed that UV treatment is more suitable for the PDMS samples used in the cell culture experiments. Graphical Abstract: UF0001 Novelty Statement: PDMS is a widely used polymer for cell culture applications which required surface treatments and sterilization of PDMS. In our study, we evaluated surface treatments and sterilization techniques on the mechanical properties of PDMS and on the cells growing on the PDMS in relation with oxidative stress metabolism and cell nutrient consumption of cells that have not been studied before. Our data have revealed an optimized and effective method for the usage of PDMS for biological applications. HIGHLIGHTS: PDMS samples were treated with UV, ethanol, plasma oxidation, cell media, cells and collagen. UV sterilization showed the best outcome in terms of biocompatibility of PDMS samples. Data revealed that UV-treated PDMS samples promote cell proliferation and adhesion. This optimized method may guide researchers for biological applications of PDMS used in cell culture experiments.