Chemoenzymatic synthesis of 6′-sialolactose-modified nanobody. Issue 2 (24th March 2022)

• Record Type: Journal Article
• Authors: Jiang, Min; Zhao, Jie; Mei, Guodong; Lin, Han; Hong, Haofei; Li, Dan; Wu, Zhimeng
• Is Part Of: Journal of carbohydrate chemistry. Volume 41:Issue 2/3(2022)
• Page Start: 207
• Page End: 221
• Publication Date: 2022-03-24
• Subjects: Nanobody -- Sortase A -- 6′-sialyllactose; Carbohydrates -- Periodicals; 547.78
• DOI: 10.1080/07328303.2022.2055048
• Languages: English
• ISSNs: 0732-8303

Abstract

Abstract: 6′-Sialolactose-modified mono- and bivalent nanobodies were designed and synthesized by a chemoenzymatic strategy. Epidermal growth factor receptor (EGFR)-targeting 7D12 nanobodies, including mono- and bivalent 7D12, were recombinantly expressed in Escherichia coli . Triglycine-modified 6′-sialolactose derivative was synthesized in nine steps. The conjugation of carbohydrates with nanobodies was achieved by Sortase-A mediated ligation with high conversion rates. The obtained glycoengineered nanobodies were finally verified by SDS-PAGE, western blot, and mass spectrometry. Graphical Abstract: UF0001