Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies. Issue 48 (26th May 2022)

• Record Type: Journal Article
• Authors: Du, Yuying; May, Robin C.; Cooper, Helen J.
• Is Part Of: Chemical communications. Volume 58:Issue 48(2022)
• Page Start: 6857
• Page End: 6860
• Publication Date: 2022-05-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc02085h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Electroporation combined with native liquid extraction surface analysis mass spectrometry allows analysis of intact protein assemblies directly from bacterial colonies. Abstract : Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.