A NIR fluorescent probe for the specific detection of hypochlorite and its application in vitro and in vivo. Issue 22 (25th May 2022)

• Record Type: Journal Article
• Authors: Zhen, Lu; Lan, Jinshuai; Zhang, Shengan; Liu, Li; Zeng, Ruifeng; Chen, Yi; Ding, Yue
• Is Part Of: Analytical methods. Volume 14:Issue 22(2022)
• Page Start: 2147
• Page End: 2152
• Publication Date: 2022-05-25
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d2ay00561a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A colorimetric NIR fluorescent probe for detecting ClO − has been synthesized. And it had outstanding advantages: one-step synthesis, high sensitivity, selectivity and successful application in actual water samples, living cells and zebrafish. Abstract : It is of great necessity to exploit a real-time, highly selective and sensitive method for hypochlorite (ClO − ) detection in both the environment and living systems because of the complex influence of ClO − on health. In this paper, based on the intramolecular charge transfer (ICT) effect, a NIR fluorescent probe (probe DAB) was designed for the accurate detection of ClO −, which produced a fluorescence response to ClO − with high selectivity and rapid response (within 1 min). The probe DAB could determine ClO − over the linear range of 0–80 μM with a low detection limit of 1.46 μM. And the sensing mechanism between the probe and ClO − was verified using HPLC and MS. To further prove its practicability, the probe was applied for detecting ClO − in actual water samples. In addition, owing to its good sensing properties and low cytotoxicity, probe DAB could be expediently applied to visualize ClO − in living cells and zebrafish, and it is expected to be a useful tool for investigating the detailed functions and mechanisms of ClO − in living systems.