Analysis of cell–nanoparticle interactions and imaging of in vitro labeled cells showing barcorded endosomes using fluorescent thiol-organosilica nanoparticles surface-functionalized with polyethyleneimine. Issue 12 (25th May 2022)

• Record Type: Journal Article
• Authors: Nakamura, Michihiro; Nakamura, Junna; Mochizuki, Chihiro; Kuroda, Chika; Kato, Shigeki; Haruta, Tomohiro; Kakefuda, Mayu; Sato, Shun; Tamanoi, Fuyuhiko; Sugino, Norihiro
• Is Part Of: Nanoscale advances. Volume 4:Issue 12(2022)
• Page Start: 2682
• Page End: 2703
• Publication Date: 2022-05-25
• Subjects: 620.5
• DOI: 10.1039/d1na00839k
• Languages: English
• ISSNs: 2516-0230

Abstract

Abstract : "Endosomal barcoded cells" via the differential intraendosomal sorting of particles depending on the particle surface were prepared using simultaneous dual-particle administration and observed on seamless biomedical imaging. Abstract : Biomedical imaging using cell labeling is an important technique to visualize cell dynamics in the body. To label cells, thiol-organosilica nanoparticles (thiol-OS) containing fluorescein (thiol-OS/Flu) and rhodamine B (thiol-OS/Rho) were surface-functionalized with polyethyleneimine (PEI) (OS/Flu-PEI and OS/Rho-PEI) with 4 molecular weights (MWs). We hypothesized PEI structures such as brush, bent brush, bent lie-down, and coiled types on the surface depending on MWs based on dynamic light scattering and thermal gravimetric analyses. The labeling efficacy of OS/Flu-PEIs was dependent on the PEI MW and the cell type. A dual-particle administration study using thiol-OS and OS-PEIs revealed differential endosomal sorting of the particles depending on the surface of the NPs. The endosomes in the labeled cells using OS/Flu-PEI and thiol-OS/Rho revealed various patterns of fluorescence termed barcoded endosomes. The cells labeled with OS-PEI in vitro were administrated to mice intraperitoneally after in situ labeling of peritoneal cells using thiol-OS/Rho. The in vitro labeled cells were detected and identified in cell aggregates in vivo seamlessly. The labeled cells with barcoded endosomes were also identified in cell aggregates. Biomedical imaging of in vitro OS-PEI-labeled cells combined with in situ labeled cells showed high potential for observation of cell dynamics.