Biodistribution of 89Zr-oxine-labeled human bone marrow-derived mesenchymal stem cells by micro-PET/computed tomography imaging in Sprague–Dawley rats. Issue 7 (19th April 2022)

• Record Type: Journal Article
• Authors: Wang, Shuzhe; Wang, Yan; Xu, Bohua; Qin, Tian; Lv, Yupeng; Yan, Heng; Shao, Yifei; Fang, Yangyang; Zheng, Shaoqiu; Qiu, Yunliang
• Is Part Of: Nuclear medicine communications. Volume 43:Issue 7(2022)
• Page Start: 834
• Page End: 846
• Publication Date: 2022-04-19
• Subjects: 89Zr-oxine -- biodistribution -- cell tracking -- human bone marrow-derived mesenchymal stem cells -- micro-PET–computed tomography -- radiolabeling; Nuclear medicine -- Periodicals; 616.07575
• DOI: 10.1097/MNM.0000000000001562
• Languages: English
• ISSNs: 0143-3636

Abstract

Abstract : Purpose: To develop a method for labeling human bone marrow mesenchymal stem cells (hMSCs) with 89 Zr-oxine to characterize the biodistribution characteristics of hMSCs in normal Sprague–Dawley (SD) rats in real-time by micro-PET–computed tomography (micro-PET/CT) imaging. Methods: 89 Zr-oxine complex was synthesized from 89 Zr-oxalate and 8-hydroxyquinoline (oxine). After hMSCs were labeled with the 89 Zr-oxine complex, the radioactivity retention, viability, proliferation, apoptosis, differentiation, morphology, and phenotype of labeled cells were assessed. The biodistribution of 89 Zr-oxine-labeled hMSCs in SD rats was tracked in real-time by micro-PET/CT imaging. Results: The cell labeling efficiency was 52.6 ± 0.01%, and 89 Zr-oxine was stably retained in cells (66.7 ± 0.9% retention on 7 days after labeling). Compared with the unlabeled hMSCs, 89 Zr-oxine labeling did not affect the biological characteristics of cells. Following intravenous administration in SD rats, labeled hMSCs mainly accumulated in the liver (7.35 ± 1.41% ID/g 10 days after labeling, n = 6) and spleen (8.48 ± 1.20% ID/g 10 days after labeling, n = 6), whereas intravenously injected 89 Zr-oxalate mainly accumulated in the bone (4.47 ± 0.35% ID/g 10 days after labeling, n = 3). Conclusion: 89 Zr-oxine labeling and micro-PET/CT imaging provide a useful and non-invasive method of assessing the biodistribution of cell therapy products in SD rats. The platform provides a foundation for us to further understand the mechanism of action and migration dynamics of cell therapy products.