Functional improvement of (−)-epicatechin gallate and piceatannol through combined binding to β-lactoglobulin: Enhanced effect of heat treatment and nanoencapsulation. (July 2022)

• Record Type: Journal Article
• Authors: Ren, Yongfang; Liu, Tingting; Liu, He; Zhu, Yihao; Qi, Xin; Liu, Xiaofan; Zhao, Yanna; Wu, Yushu; Zhang, Ning; Liu, Min
• Is Part Of: Journal of functional foods. Volume 94(2022)
• Publication Date: 2022-07
• Subjects: Polyphenol -- β-lactoglobulin -- Heat treatment -- Nanoencapsulation -- Synergistic effect; β-LG β-lactoglobulin -- ECG (−)-epicatechin gallate -- PIC piceatannol; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105120
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Combined binding of ECG and PIC to β-lactoglobulin was studied. Heat treatment enhanced the binding affinity between ECG/PIC and β-LG. Synergistic antioxidant and cytotoxic activity between ECG and PIC was verified. H-β-LG nanoparticles improved the stability, bioaccessibility and cytotoxicity. Abstract: Several polyphenol compounds commonly found in plant foods provide health benefits that promote their applications in food and pharmaceutical fields. The co-loading of proteins can improve the biological activity and synergy of combined polyphenols. Herein, multi-spectroscopic techniques and molecular docking were employed to investigate the interactions of native/heat treated β-lactoglobulin (β-LG/H-β-LG) with (−)-epicatechin gallate (ECG) and/or piceatannol (PIC). Furthermore, the synergistic antioxidant activity and cytotoxicity of ECG and PIC were demonstrated. The loading of β-LG/H-β-LG protected the antioxidant activity of ECG and PIC in the delivery systems and enhanced their cytotoxicity, stability, and bioaccessibility. Compared to the corresponding complexes, the nanoparticles of β-LG/H-β-LG had a greater impact on the functional properties and synergistic effects of ECG and PIC. The experimental results may provide a theoretical basis for the practical application of ECG and PIC co-loaded by H-β-LG, particularly H-β-LG nanoparticles in the food and pharmaceutical industries.