Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1 → 4)-glucosidic segments of various lengths. (1st September 2022)

• Record Type: Journal Article
• Authors: Lang, Weeranuch; Kumagai, Yuya; Habu, Shinji; Sadahiro, Juri; Tagami, Takayoshi; Okuyama, Masayuki; Kitamura, Shinichi; Sakairi, Nobuo; Kimura, Atsuo
• Is Part Of: Carbohydrate polymers. Volume 291(2022)
• Publication Date: 2022-09-01
• Subjects: DDase dextran dextrinase -- DP average value of degree of polymerization -- Gn maltodextrin with DP = n -- IMS isomaltomegalosaccharide -- IMS-p/q (e.g., IMS-15/9) IMS with DPs of p and q for α-(1 → 6)- and α-(1 → 4)-segments, respectively -- Kc stability constant -- Ks equilibrium constant -- MWCO-2000 and MWCO-3500 dialysis tubes with molecular weight cutoffs of 2000 and 3500, respectively -- QC quercetin -- Q3G quercetin-3-O-β-glucoside -- TDT thermal decomposition temperature -- TGA thermogravimetric analysis -- TNS 2-p-toluidinylnaphthalene-6-sulfonate; Dextran dextrinase -- Isomaltomegalosaccharide -- Chimeric structure -- Bifunctionality -- Quercetin flavonoid -- Water solubility enhancement; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119562
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Isomaltomegalosaccharide (IMS) is a long chimeric glucosaccharide composed of α-(1 → 6)- and α-(1 → 4)-linked segments at nonreducing and reducing ends, respectively; the hydrophilicity and hydrophobicity of these segments are expected to lead to bifunctionality. We enzymatically synthesized IMS with average degrees of polymerization (DPs) of 15.8, 19.3, and 23.5, where α-(1 → 4)-segments had DPs of 3, 6, and 9, respectively. IMS exhibited considerably higher water solubility than maltodextrin because of the α-(1 → 6)-segment and an identical resistance to thermal degradation as short dextran. Interaction of IMS with a fluorescent probe of 2- p -toluidinylnaphthalene-6-sulfonate demonstrated that IMS was more hydrophobic than maltodextrin, where the degree of hydrophobicity increased as DP of α-(1 → 4)-segment increased (9 > 6 > 3). Fluorescent pyrene-estimating polarity of IMS was found to be similar to that of methanol or 1-butanol. The bifunctional IMS enhanced the water solubility of quercetin-3- O -glucoside and quercetin: the solubilization of less-soluble bioactive substances is beneficial in carbohydrate industry. Graphical abstract: Unlabelled Image