New sight into interaction between endoplasmic reticulum stress and autophagy induced by vanadium in duck renal tubule epithelial cells. (1st August 2022)

• Record Type: Journal Article
• Authors: Wang, Li; Pan, Yueying; Yang, Fan; Guo, Xiaowang; Peng, Junjun; Wang, Xiaoyu; Fang, Yukun; Chen, Jing; Yi, Xin; Cao, Huabin; Hu, Guoliang
• Is Part Of: Chemico-biological interactions. Volume 362(2022)
• Publication Date: 2022-08-01
• Subjects: Vanadium -- Endoplasmic reticulum stress -- Autophagy -- Renal tubular epithelial cells -- Duck; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2022.109981
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Vanadium (V) is a common environmental and industrial pollutant that can cause nephrotoxicity in animals in excess. The purpose of this research was to explore the interaction between endoplasmic reticulum (ER) stress and autophagy induced by V in the kidney of ducks. Duck renal tubule epithelial cells were exposed to different concentrations of sodium metavanadate (NaVO3 ) (0, 100 and 200 μM) and PERK inhibitor (GSK, 1 μM), or autophagy inhibitor (chloroquine, 50 μM) alone for 24 h (chloroquine for the last 4 h). The results showed that exposure to V caused the dilatation and swelling of the ER and intracellular calcium overload, and upregulated PERK, eIF2α, ATF4 and CHOP mRNA levels and p-PERK and CHOP protein levels associated with ER stress in cells. Additionally, V markedly increased the number of autophagosomes, acidic vesicular organelles (AVOs) and LC3 puncta, as well as the mRNA levels of Beclin1, Atg5, Atg12, LC3A and LC3B and protein levels of Beclin1, Atg5 and LC3B-II/LC3B–I, but decreased the imRNA and protein levels of p62. Moreover, treatment with the PERK inhibitor ameliorated the changed factors above induced by V, but the V-induced variation of ER-stress related factors were aggravated after treatment with the autophagy inhibitor. Together, our data suggested that excessive V could induce ER stress and autophagy in duck renal tubular epithelial cells. ER stress might promote V-induced autophagy via the PERK/ATF4/CHOP signaling pathway, and autophagy may play a role in alleviating ER stress induced by V. Graphical abstract: Image 1 Highlights: Vanadium (V) has toxic effect on duck renal tubular epithelial cells. V could induce endoplasmic reticulum (ER) stress via PERK/ATF4/CHOP pathway. V could trigger autophagy in duck renal tubular epithelial cells. ER stress might promote V-induced autophagy, and autophagy may play a role in alleviating ER stress induced by V.