Anti-tumor and immunomodulation activity of polysaccharides from Dendrobium officinale in S180 tumor-bearing mice. (July 2022)

• Record Type: Journal Article
• Authors: Sun, Chuanbo; Zhang, Nuo; Xu, Guangpei; Jiang, Ping; Huang, Shiping; Zhao, Qun; He, Yanfei
• Is Part Of: Journal of functional foods. Volume 94(2022)
• Publication Date: 2022-07
• Subjects: Anti-tumor -- Immunomodulation activity -- Polysaccharides -- Dendrobium officinale; CTL Cytotoxic T lymphocyte -- CDOP Crude Dendrobium officinale polysaccharides -- CTX Cyclophosphamide -- DOP Polysaccharides from Dendrobium officinale -- ELISA Enzyme-linked immunosorbent assay -- FT-IR Fourier Transform Infrared Spectroscopy -- GC–MS Gas chromatography Mass spectrum -- H&E Hematoxylin and Eosin -- HPGPC High-Performance Gel Permeation Chromatography -- IL-10 Interleukin-10 -- IFN-γ Interferon-γ -- IgG Immunoglobulins G -- IgM Immunoglobulins M -- NMR Nuclear Magnetic Resonance -- NK cell Natural killer cell -- TMB Tetramethyl benzidine; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.105105
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: DOP consisted of D-mannose, D-glucose, and D-galactose in a mole ratio of 3:2:1. DOP possesses antitumor and immunomodulation activity. DOP enhanced NK cell and CTL activity and increased the non-specific and tumor antigen-specific antibody titers. DOP could inhibit the growth of S180 tumors by improving the tumor immune microenvironment. Abstract: This study aims to explore the structural features and anti-tumor and immunomodulation activity of polysaccharides from Dendrobium officinale that were prepared using DEAE Sephadex A-50 and Sephadex G100. DOP structure was characterized using colorimetry, HPGPC, GC–MS, FT-IR, and NMR. The molecular weight of DOP was 1.89 × 10 5 Da. And the DOP consisted of D-mannose, D-glucose, and D-galactose in a mole ratio of 3:2:1. DOP could inhibit the growth of S180 tumors by promoting splenocytes proliferation, enhancing NK cell and CTL activity, and increasing the non-specific and tumor antigen-specific antibody levels. These findings suggested that DOP might be a potential antitumor agent with immunomodulatory activity.